姜黄素,一种来自姜黄的天然生物活性化合物,已被广泛认可的抗真菌特性。在这项研究中,我们研究了姜黄素对植物病原真菌Alternariaalternata的影响及其在樱桃番茄果实中的致病性。结果表明,姜黄素处理以剂量依赖的方式显着抑制了交替草的菌丝生长和孢子萌发。扫描电子显微镜显示用姜黄素处理的交替草菌丝体的形态发生变化。此外,姜黄素处理导致丙二醛和过氧化氢含量增加,表明A.alternata的细胞膜损伤。此外,姜黄素对樱桃番茄果实黑腐病的发生和病变直径具有明显的抑制作用。基因表达分析显示防御相关基因上调(POD,SOD,和CAT)在用姜黄素处理的番茄果实中。此外,姜黄素治疗导致细胞外致病酶(聚半乳糖醛酸酶,果胶裂解酶,和内-1,4-β-d-葡聚糖酶)在A.alternata中。总的来说,我们的发现强调了姜黄素作为一种有效的抗真菌药物的潜力,提供对其菌丝生长抑制机制的见解,孢子萌发,樱桃番茄果实的致病性。
Curcumin, a natural bioactive compound derived from Curcuma longa, has been widely recognized for its antifungal properties. In this study, we investigated the effects of curcumin on the phytopathogenic fungus Alternaria alternata and its pathogenicity in cherry tomato fruit. The results demonstrated that curcumin treatment significantly inhibited mycelial growth and spore germination of A. alternata in a dose-dependent manner. Scanning electron microscopy revealed alterations in the morphology of A. alternata mycelia treated with curcumin. Furthermore, curcumin treatment led to an increase in malondialdehyde and hydrogen peroxide contents, indicating cell membrane damage in A. alternata. Moreover, curcumin exhibited a remarkable inhibitory effect on the incidence and lesion diameters of black rot caused by A. alternata in cherry tomato fruit. Gene expression analysis revealed upregulation of defense-related genes (POD, SOD, and CAT) in tomato fruit treated with curcumin. Additionally, curcumin treatment resulted in decreased activity of exocellular pathogenic enzymes (polygalacturonase, pectin lyase, and endo-1,4-β-d-glucanase) in A. alternata. Overall, our findings highlight the potential of curcumin as an effective antifungal agent against A. alternata, providing insights into its inhibitory mechanisms on mycelial growth, spore germination, and pathogenicity in cherry tomato fruit.