染色体不稳定性(CIN)在几种人类癌症的发生中起着关键作用,并且可能与纺锤体组装检查点(SAC)的核心成分(包括BUBR1蛋白激酶)的失调有关。这些蛋白质与人类口腔鳞状细胞癌(OSCC)患者的肿瘤发展和低生存率有关。为了研究SAC蛋白BUBR1,BUB3和SPINDLY以及Ki-67在犬OSCC中的表达,我们对60例犬OSCCs进行了免疫组织化学评估,并将其与临床和病理变量进行了比较.在所有病例中均检测到BUBR1,Ki-67,BUB3和SPINDLY蛋白的表达,并在BUBR131例(51.7%),BUB333例(58.9%)和28例(50.9%)中分为高表达程度评分SPINDLY。Ki-67高表达14例(25%)。发现了BUBR1的独立预后价值,其中高BUBR1表达与较低的生存率相关(p=0.012)。这些结果表明,BUBR1表达是这些肿瘤的独立预后因素。提示在犬OSCC中作为预后生物标志物和药理学靶标的临床应用的潜在用途。
Chromosomal instability (CIN) plays a key role in the carcinogenesis of several human cancers and can be related to the deregulation of core components of the spindle assembly checkpoint (SAC) including BUBR1 protein kinase. These proteins have been related to tumor development and poor survival rates in human patients with oral squamous cell carcinoma (OSCC). To investigate the expression of the SAC proteins BUBR1, BUB3 and
SPINDLY and also Ki-67 in canine OSCC, we performed an immunohistochemical evaluation in 60 canine OSCCs and compared them with clinical and pathological variables. BUBR1, Ki-67, BUB3 and
SPINDLY protein expressions were detected in all cases and classified as with a high-expression extent score in 31 (51.7%) cases for BUBR1, 33 (58.9%) cases for BUB3 and 28 (50.9%) cases for
SPINDLY. Ki-67 high expression was observed in 14 (25%) cases. An independent prognostic value for BUBR1 was found, where high BUBR1 expression was associated with lower survival (p = 0.012). These results indicate that BUBR1 expression is an independent prognostic factor in these tumors, suggesting the potential use for clinical applications as a prognostic biomarker and also as a pharmacological target in canine OSCC.