目的：衰老是男性不育的原因之一，在生物老化过程中，睾丸中存在异常的全局DNA甲基化和印迹缺陷。一种重要的新兴方法旨在利用年轻血浆的愈合特性来限制体内各种器官的衰老进程。我们的目的是显示血浆转移是否对DNA甲基化和生精细胞发育有影响。此外,我们的目的是显示年轻的血浆转移给老年小鼠是否对老年小鼠的复兴有影响，以及老年受损的DNA甲基化和PCNA表达是否可以恢复。
方法：组是（i）年轻对照组，(ii)年轻血浆向老年人转移，(iii)年龄控制，(iv)将老年血浆转移至青年。我们利用IHC和WB在Dnmts的蛋白质水平。对于全球DNA甲基化水平，我们使用5-甲基胞嘧啶染色。我们还通过IHC分析了所有组中的PCNA蛋白表达。
结果：我们发现，将年轻的血浆注入老年动物体内可以恢复DNA甲基化和PCNA表达，就像在年轻动物体内一样。最重要的是,我们观察到年轻的血浆转移后，老年动物的精原细胞和精子细胞数量增加。
结论：我们的研究结果表明，年轻的血浆移植可以恢复随着衰老而发生的表观遗传疾病，并通过增加减少的精子数量来解决不育问题。它需要得到不同研究的支持，尤其是人类研究。
OBJECTIVE: Aging is one of the causes of male infertility, and abnormal global DNA methylation and imprinting defects have been characterized in testis during biological aging. One of the important emerging approaches aims to take advantage of the healing properties of young blood plasma to limit the progression of aging in various organs in the body. We aimed to show whether blood plasma transfer has an effect on DNA methylation and spermatogenetic cell development. In addition, we aimed to show whether the young plasma transfer to old mice has an effect on the rejuvenation of the old and whether the impaired DNA methylation and PCNA expression in old age can be restored.
METHODS: Groups were (i) young control, (ii) young plasma transfer to aged, (iii) aged control, (iv) aged plasma transfer to young. We utilized IHC and WB in protein level of Dnmts. For the global DNA methylation level, we used 5-methylcytosine staining. We also analyzed PCNA protein expressions in all groups by IHC.
RESULTS: We found that transfusion of young plasma into the old animal restored DNA methylation and PCNA expression as it did in the young animal. Most importantly, we observed an increase in spermatogonia and spermatid counts in older animals after young blood plasma transfer.
CONCLUSIONS: Our findings show that young plasma transfer can restore epigenetic disorders that occur with aging and solve infertility problems by increasing the sperm count that decreases. It needs to be supported by different studies, especially human studies.
