背景:猫骨关节炎(OA)导致慢性疼痛和体感致敏。在人类中,感官暴露可以调节慢性疼痛。最近,脑电图(EEG)揭示了人类OA的特定大脑特征。然而,在OA猫中不存在EEG疼痛表征或其调制,所有的脑电图都是在镇静的猫身上进行的,使用皮内电极,这可以改变感觉(疼痛)知觉。
方法:使用十个镀金表面电极评估受OA影响的猫(n=11)。感觉刺激以随机顺序呈现:对机械时间总和的反应,葡萄柚气味和单色波长(500nm蓝色,525nm-绿光和627nm-红光)。对记录的EEG进行处理以识别事件相关电位(ERP)并进行频谱分析(z分数)。
结果:手术耐受性良好。报告了两种机械的ERP(F3、C3、Cz、P3,Pz)和嗅觉刺激(Cz,Pz).主要限制是运动伪影。光谱分析显示EEG频带的功率与光波长之间存在显著的相互作用(p<0.001)。考虑所有波长,α带比例高于δ带和γ带(p<0.044),而后者低于β带(p<0.016)。与绿色和红色相比,暴露于蓝光引起脑电图功率随时间的明显变化(p<0.001)。
结论:这是有意识的猫的EEG可行性的首次证明,该猫具有表面电极记录大脑活动,同时将其暴露于感官刺激。
结论:ERPs和光谱模式的鉴定为研究猫慢性疼痛及其通过感觉干预的潜在调节开辟了新的途径。
BACKGROUND: Feline osteoarthritis (OA) leads to chronic pain and somatosensory sensitisation. In humans, sensory exposure can modulate chronic pain. Recently, electroencephalography (EEG) revealed a specific brain signature to human OA. However, EEG pain characterisation or its modulation does not exist in OA cats, and all EEG were conducted in sedated cats, using intradermal electrodes, which could alter sensory (pain) perception.
METHODS: Cats (n=11) affected by OA were assessed using ten gold-plated surface electrodes. Sensory stimuli were presented in random orders: response to mechanical temporal summation, grapefruit scent and mono-chromatic wavelengths (500 nm-blue, 525 nm-green and 627 nm-red light). The recorded EEG was processed to identify event-related potentials (ERP) and to perform spectral analysis (z-score).
RESULTS: The procedure was well-tolerated. The ERPs were reported for both mechanical (F3, C3, Cz, P3, Pz) and olfactory stimuli (Cz, Pz). The main limitation was motion artifacts. Spectral analysis revealed a significant interaction between the power of EEG frequency bands and light wavelengths (p<0.001). All wavelengths considered, alpha band proportion was higher than that of delta and gamma bands (p<0.044), while the latter was lower than the beta band (p<0.016). Compared to green and red, exposure to blue light elicited distinct changes in EEG power over time (p<0.001).
CONCLUSIONS: This is the first demonstration of EEG feasibility in conscious cats with surface electrodes recording brain activity while exposing them to sensory stimulations.
CONCLUSIONS: The identification of ERPs and spectral patterns opens new avenues for investigating feline chronic pain and its potential modulation through sensory interventions.