由于胃癌的高转移性和异质性,目前尚无有效的治疗方法。据报道,罗哌卡因(Rop)可以抑制生长,迁移,和胃癌的侵袭。然而,Rop的治疗机制仍需进一步探讨,为其临床应用提供见解。本研究旨在探讨罗普对其生长的影响,迁移,和胃癌细胞的侵袭和潜在机制。通过qRT-PCR评估SNX10在胃癌组织和细胞系AGS中的表达水平。细胞计数试剂盒-8(CCK8)测定,伤口愈合试验,然后使用transwell测定法来检查Rop对AGS细胞活力的影响,迁移,入侵,和扩散,分别。此外,集落形成试验用于测量细胞增殖能力,流式细胞术检测细胞凋亡水平。SNX10、SRC、通过蛋白质印迹检测STAT3。根据实验结果,在胃癌组织和AGS细胞系中观察到SNX10mRNA表达降低。Rop抑制了增殖,迁移,和AGS细胞的入侵,但促进细胞凋亡和上调SNX10表达。此外,Rop抑制MMP-2和MMP-9的表达,抑制SRC和STAT3的磷酸化。SNX10敲低可以逆转Rop诱导的抗癌作用。总的来说,Rop在预防胃癌的增殖和转移中显示出潜在的作用。其作用机制可能与上调SNX10的表达和进一步抑制SRC/STAT3信号通路有关。我们的发现为Rop的抗癌特性提供了新的见解。
Gastric cancer currently has no effective treatment due to its high metastasis and heterogeneity. It has been reported that ropivacaine (Rop) can inhibit the growth, migration, and invasion of gastric cancer. However, the therapeutic mechanism of Rop still needs to be further explored to provide insights for its clinical application. This study aimed to explore the effects of Rop on the growth, migration, and invasion of gastric cancer cells and the underlying mechanisms. The expression levels of SNX10 were assessed in gastric cancer tissues and cell line AGS by qRT-PCR. Cell Counting Kit-8 (CCK8) assay, wound-healing assay, and transwell assay were then used to examine the effects of Rop on the AGS cell viability, migration, invasion, and proliferation, respectively. Additionally, colony formation assay was used to measure cell proliferation ability, and flow cytometry was used to detect apoptosis level. Protein levels of SNX10, SRC, and STAT3 were detected by western blot. According to the experimental results, the decreased SNX10 mRNA expression was observed in gastric cancer tissue and cell line AGS. Rop inhibited the proliferation, migration, and invasion of AGS cells, but promoted apoptosis and upregulated SNX10 expression. Moreover, Rop inhibited the expression of MMP-2 and MMP-9, phosphorylation of SRC and STAT3. SNX10 knockdown could reverse Rop-induced anticancer effects. Collectively, Rop showed a potential role in preventing proliferation and metastasis of gastric cancer. The action mechanism of Rop may be related to the upregulation of SNX10 expression and further inhibition of SRC/STAT3 signaling pathway. Our findings provide new insights into the anticancer properties of Rop.