研究了在中温条件下用零价铁粉(ZVI)增强的原位高级厌氧消化(AAD)去除5种抗生素(磺胺erazine(SMR),磺胺甲恶唑(SMZ)氧氟沙星(OFL),四环素(TC),和罗红霉素(ROX)和11种抗生素抗性基因(ARGs)(AAC(6')-IB-CR,qnrS,ermF,ermT,ermX,sul1,sul2,sul3,tetA,tetB,和tetG)在污水污泥中。不同ZVI剂量的影响,抗生素浓度,并探讨了固体保留时间(SRT)对去除效果的影响。此外,抗生素和ARGs的相关系数,微生物群落结构,分析了沼气产量和甲烷产量。所有进行的处理运行稳定,改进的Gompertz模型很好地描述了累积甲烷产量。抗生素,除了OFL,在1000mg/LZVI的投加量下有效去除污水污泥,SRT20d,AAD期间抗生素浓度为20μg/L。SMZ的去除率,SMR,TC,ROX达到97.39%,74.54%,78.61%,和56.58%,分别。在原位AAD过程中,AAC(6')-IB-CR和tetB可以有效降低。通过冗余分析,AAC(6')-IB-CR,ermT,ermX,sul2,tetB,tetG与反应器中的抗生素有很强的正相关关系。主成分分析表明,在污泥中ZVI和抗生素浓度相同的情况下,SRT为10d和20d时,群落结构相似。在1000mg/LZVI投加量的操作参数下,SRT20d,抗生素浓度为20μg/L,Erysipelotrichia,Verrucomicrobia,梭菌,Caldiserica,类的α-变形杆菌是厌氧消化中占主导地位的微生物。
The in-situ advanced anaerobic digestion (AAD) enhanced with zero-valent iron powder (ZVI) under mesophilic condition was investigated to remove 5 antibiotics (sulfamerazine (SMR), sulfamethoxazole (SMZ), ofloxacin (OFL), tetracycline (TC), and roxithromycin (ROX)) and 11 antibiotic resistance genes (ARGs) (AAC (6\')-IB-CR, qnrS, ermF, ermT, ermX, sul1, sul2, sul3, tetA, tetB, and tetG) in sewage sludge. The effects of different ZVI dosages, antibiotic concentrations, and solid retention time (SRTs) on the removal were explored. Also, the correlation coefficient of antibiotics and ARGs, microbial community structure, biogas production and methane yield were analyzed. All conducted treatments operated stably, and the modified Gompertz model described the cumulative methane yield well. The antibiotics, with the exception of OFL, were effectively removed in the sewage sludge at a dosage of 1000 mg/L ZVI, SRT 20 d, and an antibiotic concentration of 20 μg/L during AAD. The removal rates of SMZ, SMR, TC, and ROX reached 97.39%, 74.54%, 78.61%, and 56.58%, respectively. AAC (6\')-IB-CR and tetB could be effectively reduced during the in-situ AAD. Through the redundancy analysis, AAC (6\')-IB-CR, ermT, ermX, sul2, tetB, and tetG had strong positive correlations with the antibiotics in the reactor. The principle component analysis revealed that the community structure was similar when the SRT was 10 d and 20 d at the same amount of ZVI and antibiotic concentrations in the sludge. Under the operating parameters of 1000 mg/L ZVI dosage, SRT 20 d, and an antibiotic concentration of 20 μg/L, Erysipelotrichia, Verrucomicrobia, Clostridia, Caldiserica, and Alphaproteobacteria of the class were dominated microorganisms in the anaerobic digestion.