钩藤是我国重要的传统中药材,通过改变土壤性质,选择合适的土壤改良剂可以提高钩藤的产量和品质。在本文中,研究了钩藤相关生物碱和土壤微生物群落。田间试验设置了以下对照组:(M1,无土壤调理剂)和不同土壤调理剂处理组(M2,生物量灰分;M3,保水剂;M4,生物炭;M5,石灰粉和M6,苹果酸)。结果表明,M2显著增加了肉的鲜重和干重以及异钩藤碱的含量,Corynoxeine,异可可碱,和总生物碱。酸杆菌,变形杆菌,放线菌,氯氟菌是主要的细菌门。相关性分析表明,鲜重和干重与酸性细菌呈极显著正相关,而碱水解氮,磷酸酶活性,新鲜和干重,Corynoxeine,异曲尼与氯氟菌呈显著负相关。土壤调理剂M2的施用增加了酸细菌的丰度,降低了氯氟的丰度,这有助于提高土壤养分含量,产量,和钩藤的质量。总之,生物量灰分可能是钩藤种植区土壤调理剂的更好选择。
Uncaria rhynchophylla is an important traditional herbal medicine in China, and the yield and quality of Uncaria rhynchophylla can be improved by suitable soil conditioners because of changing the soil properties. In this paper, Uncaria rhynchophylla associated alkaloids and soil microbial communities were investigated. The field experiment was set up with the following control group: (M1, no soil conditioner) and different soil conditioner treatment groups (M2, biomass ash; M3, water retention agent; M4, biochar; M5, lime powder and M6, malic acid). The results showed that M2 significantly increased the fresh and dry weight and the contents of isorhynchophylline, corynoxeine, isocorynoxeine, and total alkaloids. Acidobacteria, Proteobacteria, Actinobacteria, and Chloroflexi were major bacterial phyla. Correlation analysis showed that fresh and dry weight was significantly positively correlated with Acidobacteria, while alkali-hydrolyzable nitrogen, phosphatase activity, fresh and dry weight, corynoxeine, and isocorynoxeine were significantly negatively correlated with Chloroflexi. The application of soil conditioner M2 increased the abundance of Acidobacteria and decreased the abundance of Chloroflexi, which contributed to improving the soil nutrient content, yield, and quality of Uncaria rhynchophylla. In summary, biomass ash may be a better choice of soil conditioner in Uncaria rhynchophylla growing areas.