目的:评价细颗粒物(PM2.5)和臭氧(O3)联合染毒对SD大鼠鼻黏膜三磷酸腺苷(ATP)含量和ATP酶活性的影响。
方法:20只雄性SD大鼠按随机数字表法分为对照组(n=10)和染毒组(n=10)。老鼠在常规清洁环境和我们团队建立的空气污染物暴露系统中喂养,分别,并暴露了208天。在暴露期间,监测暴露系统中PM2.5和O3的浓度,结合自测和现场数据,对暴露系统中的PM2.5和O3进行了综合评估。在208d的曝光中,核心,肝脏,脾,脾肾,收集大鼠的睾丸和其他主要器官以及鼻粘膜组织。称重每个器官并计算器官系数。通过生物发光测量ATP的总量,分光光度法检测Na+-K+-ATP酶和Ca2+-ATP酶的活性。两个独立样本的t检验用于比较指标组之间的差异。
结果:从第3周到暴露时间结束,暴露组大鼠体重高于对照组(P<0.05),两组脏器系数差异无统计学意义。暴露组PM2.5日平均浓度为(30.68±19.23)μg/m3,最高8h臭氧浓度(O3-8h)为(82.45±35.81)μg/m3。染毒组大鼠鼻黏膜ATP化学发光值(792.4±274.1)IU/L低于对照组(1126.8±218.1)IU/L。染毒组大鼠鼻黏膜Na+-K+-ATPase活性(1.53±0.85)U/mg低于对照组(4.31±1.60)U/mg(P<0.05)。对照组和暴露组鼻黏膜蛋白含量分别为(302.14±52.51)mg/L和(234.58±53.49)mg/L,分别,Ca2+-ATP酶活性分别为(0.81±0.27)U/mg和(0.99±0.73)U/mg,分别。组间无显著差异。
结论:在PM2.5和O3亚慢性低浓度暴露下,大鼠鼻粘膜的功率容量能力降低。
OBJECTIVE: To evaluate the effects of fine particle matter (PM2.5) and ozone (O3) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats.
METHODS: Twenty male SD rats were divided into control group (n=10) and exposure group (n=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM2.5 and O3 in the exposure system were monitored, and a comprehensive assessment of PM2.5 and O3 in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na+-K+ -ATPase and Ca2+ -ATPase were detected by spectrophotometry. The t test of two independent samples was used to compare the differences among the indicator groups.
RESULTS: From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (P < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM2.5 concentration in the exposure group was (30.68±19.23) μg/m3, and the maximum 8 h ozone concentration (O3-8 h) was (82.45±35.81) μg/m3. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na+-K+-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (P < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca2+-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups.
CONCLUSIONS: The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM2.5 and O3.