目的:本研究的目的是研究潜在的抗氧化剂,抗炎,乙酸钠(ACE)的精子功能保护特性,组蛋白去乙酰化酶(HDAC)抑制剂,在大鼠睾丸扭转/扭曲(T/D)模型中。
方法:对相同体重的LittermateWistar大鼠进行假手术或睾丸T/D,方法是将左睾丸绕其轴沿精索顺时针旋转720°并将其固定在该位置两个半小时。扭曲前1小时,T/D+ACE处理的大鼠用ACE处理(200mg/kg/天,每个os),而T/D大鼠通过施用0.5mL蒸馏水进行媒介物处理。72小时后,动物被安乐死,收集左睾丸进行生物分子和组织学分析。
结果:醋酸盐给药减弱了T/D诱导的血清和睾丸HDAC和睾丸黄嘌呤氧化酶的升高,尿酸,MDA,GSSG,MPO,TNF-α,IL-1β,IL-6,NFkB,HIF-1α,和VCAM-1。此外,醋酸盐治疗缓解了T/D诱导的精子质量下降(计数,运动性,生存能力,和正常形态)和睾丸3β-HSD,17β-HSD,睾丸激素,GSH,GSH/GSSG,SOD,过氧化氢酶,GPx,GST,Nrf2和HO-1。此外,醋酸盐可防止T/D扭曲的睾丸组织结构和生精生殖细胞丢失。
结论:睾丸缺血后T/D阶段的乙酸钠可能有利于预防I/R损伤和维持生育能力。
OBJECTIVE: The aim of this study was to investigate the potential antioxidant, anti-inflammatory, and sperm function-preserving properties of sodium acetate (ACE), a histone deacetylase (HDAC) inhibitor, in a rat model of testicular torsion/detorsion (T/D).
METHODS: Littermate Wistar rats of identical weight were subjected to sham surgery or testicular T/D by rotating the left testis at 720° around its axis along the spermatic cord clockwise and fixing it in this position for two and a half hours. 1 h before detorsion, T/D + ACE-treated rats were treated with ACE (200 mg/kg/day, per os) while T/D rats were vehicle-treated by administering 0.5 mL of distilled water. After 72 h, animals were euthanized, and the left testes were harvested for bio-molecular and histological analysis.
RESULTS: Acetate administration attenuated T/D-induced rises in serum and testicular HDAC and testicular xanthine oxidase, uric acid, MDA, GSSG, MPO, TNF-α, IL-1β, IL-6, NFkB, HIF-1α, and VCAM-1. In addition, acetate treatment alleviated T/D-induced decline in sperm quality (count, motility, viability, and normal morphology) and testicular 3β-HSD, 17β-HSD, testosterone, GSH, GSH/GSSG, SOD, catalase, GPx, GST, Nrf2, and HO-1. Furthermore, acetate prevented T/D-distorted testicular histoarchitecture and spermatogenic germ cell loss.
CONCLUSIONS: Sodium acetate during the post-ischaemic phase of testicular T/D may be beneficial in preventing I/R injury and maintaining fertility.