■Maedi-visna(MV)是一种影响绵羊和山羊的小型反刍动物慢病毒(SRLV)疾病,并导致包括肺在内的各种器官的病理改变,肺淋巴结,乳腺,接头,和CNS。目的:本研究的重点是检测自发受MVV影响的绵羊和山羊的不同器官中的MV病毒(MVV)核酸和MVVp28抗原。
■总共从绵羊和山羊中收集了657个样本(169个血液,136肺,96个肺淋巴结,74大脑,54乳腺,78个接头,和50脾),并使用巢式PCR测定法筛选MVV核酸。通过cELISA筛选血清样品的SRLV抗体。通过在肺中免疫组织化学使用针对p28抗原的多克隆抗体证明了MVV的免疫定位,淋巴结,乳腺,和关节组织。
■在657个样本中,发现MVV阳性的10.7%(70)。在不同的器官中,肺部表现出最高的阳性(25.7%),其次是乳腺(14.8%),血液(9.5%),关节组织(7.7%),大脑(5.4%),和肺淋巴结(1.0%)。通过cELISA在绵羊和山羊的29.2%的血清样品中检测到SRLV抗体。在肺中观察到MVVp28抗原免疫染色,淋巴结,乳腺,和关节组织。然而,在脑组织中无法证明MVVp28抗原的存在。
■肺组织中MVV的最高阳性表明肺组织中病毒的较高好发。
UNASSIGNED: Maedi-visna (MV) is a small ruminant lentiviral (SRLV) disease affecting sheep and goats, and causes pathological alterations in various organs including lungs, pulmonary lymph nodes, mammary glands, joints, and CNS. Aims: Present study was focused to detect the MV virus (MVV) nucleic acid and MVV p28 antigen in different organs of the spontaneously MVV affected sheep and goats.
UNASSIGNED: Total of 657 samples were collected from sheep and goats (169 blood, 136 lungs, 96 pulmonary lymph nodes, 74 brain, 54 mammary gland, 78 joints, and 50 spleen) and screened for MVV nucleic acid using nested PCR assay. Serum samples were screened for SRLV antibodies by cELISA. Immunolocalization of MVV was demonstrated by using the polyclonal antibody against p28 antigen by immunohistochemistry in lungs, lymph nodes, mammary glands, and joint tissues.
UNASSIGNED: Out of 657 samples, 10.7% (70) were found positive for MVV. Among different organs, lungs showed highest positivity (25.7%) followed by mammary glands (14.8%), blood (9.5%), joint tissues (7.7%), brain (5.4%), and pulmonary lymph node (1.0%). SRLV antibodies were detected in 29.2% of the serum samples of both sheep and goats by cELISA. MVV p28 antigen immunostaining was observed in lungs, lymph nodes, mammary glands, and joint tissues. However, the presence of MVV p28 antigen could not be demonstrated in the brain tissues.
UNASSIGNED: The highest positivity of MVV in lung tissues indicated higher predilection of the virus in the pulmonary tissue.