目的:为了确认和调查病理性HFOs(pHFOs)的原因,包括波纹[80-200Hz]和快速波纹[200-600Hz],在慢波的UP-DOWN过渡期间,并且如果在癫痫发作开始区(SOZ)中由波纹时间耦合介导的信息传输被破坏,则会产生。
方法:我们从6名患者的同步宏电极和微电极记录的175.95iEEG接触小时中分离出217个总单位。在iEEG记录中确定了睡眠缓慢振荡(0.1-2Hz)时期。叠加在慢波上发生的iEEGHFO被转换为相量,并通过附近单位的最大放电相位进行调整(即,最大UP)。我们测试了是否,在SOZ,HFO和相关的动作电位(AP)在UP-DOWN过渡时更频繁地发生。我们还使用交叉相关图检查了纹波时间相关性。
结果:在SOZ的组级别,在慢波兴奋性的UP-DOWN转变期间,HFO和HFO相关的AP概率最高(p<<0.001)。在非SOZ,HFO和HFO相关AP在DOWN-UP过渡期间最高(p<0.001)。在SOZ的单位级别,15.6%和20%的单位在上升-下降过渡时(p<0.05f.d.r校正),在波动期间(科恩d=0.11-0.83)和快速波动(d=0.36-0.90)表现出更强劲的点火,分别。相比之下,同样在SOZ,6.6%(d=0.14-0.30)和8.5%(d=0.33-0.41)的机组在上升-下降过渡时的波动和快速波动期间的点火明显减少,分别。其他数据显示纹波和快速纹波时间相关性,涉及全球慢波,在海马之间,内嗅皮层,与非SOZ相比,SOZ中的海马旁回减少了>50%(N=3)。
结论:慢波兴奋性的UP-DOWN转变促进病理神经元的激活以产生pHFOs。大脑区域的波纹时间相关性可能在记忆巩固中很重要,并且在SOZ中被破坏,也许是PHFO一代。
OBJECTIVE: To confirm and investigate why pathological high-frequency oscillations (pHFOs), including ripples (80-200 Hz) and fast ripples (200-600 Hz), are generated during the UP-DOWN transition of the slow wave and if information transmission mediated by ripple temporal coupling is disrupted in the seizure-onset zone (SOZ).
METHODS: We isolated 217 total units from 175.95 intracranial electroencephalography (iEEG) contact-hours of synchronized macro- and microelectrode recordings from 6 patients. Sleep slow oscillation (.1-2 Hz) epochs were identified in the iEEG recording. iEEG HFOs that occurred superimposed on the slow wave were transformed to phasors and adjusted by the phase of maximum firing in nearby units (i.e., maximum UP). We tested whether, in the SOZ, HFOs and associated action potentials (APs) occur more often at the UP-DOWN transition. We also examined ripple temporal correlations using cross-correlograms.
RESULTS: At the group level in the SOZ, HFO and HFO-associated AP probability was highest during the UP-DOWN transition of slow wave excitability (p < < .001). In the non-SOZ, HFO and HFO-associated AP was highest during the DOWN-UP transition (p < < .001). At the unit level in the SOZ, 15.6% and 20% of units exhibited more robust firing during ripples (Cohen\'s d = .11-.83) and fast ripples (d = .36-.90) at the UP-DOWN transition (p < .05 f.d.r. corrected), respectively. By comparison, also in the SOZ, 6.6% (d = .14-.30) and 8.5% (d = .33-.41) of units had significantly less firing during ripples and fast ripples at the UP-DOWN transition, respectively. Additional data shows that ripple and fast ripple temporal correlations, involving global slow waves, between the hippocampus, entorhinal cortex, and parahippocampal gyrus were reduced by >50% in the SOZ compared to the non-SOZ (N = 3).
CONCLUSIONS: The UP-DOWN transition of slow wave excitability facilitates the activation of pathological neurons to generate pHFOs. Ripple temporal correlations across brain regions may be important in memory consolidation and are disrupted in the SOZ, perhaps by pHFO generation.