在2013年欧盟禁止对化妆品进行动物试验之后,全球对替代试验方法的兴趣与日俱增。为了开发替代测试方法,我们需要获得有关化学物质在体外和体内的毒性数据。然而,数据库有时提供有限的体内和体外化学品数据。Further,使用OECDTG439(体外皮肤刺激)生成的数据分散在差异数据库中,通过它们导航并不容易。因此,我们遵守了“皮肤刺激替代测试参考化学数据库系统(RCDS-皮肤刺激)”,一站式获取测试化学信息。我们通过收集理化性质建立了系统的RCDS-皮肤刺激,CAS编号,人类数据,以及来自海外化学品数据库(包括欧洲化学品管理局(ECHA)等)的体内(OECDTG404)数据。,和体外数据使用重建的人表皮(RhE)(OECDTG439)。因此,我们开发了RCDS-皮肤刺激,其中包含149种化学物质的信息,包括我们通过使用EpiDerm™SIT进行测试生成的数据,SkinEthic™RHE和KeraSkin™SIT。因此,根据我们的研究建立的RCDS-皮肤刺激将为化学品的安全性评估和替代测试方法的开发提供见解。
After EU ban on animal testing for cosmetics in 2013, there has been an increasing global interest in alternatives test methods. To development for alternatives test method, we need to get the toxic data about in vitro and in vivo of chemicals. However, database sometimes provide limited in vivo and in vitro data on chemicals. Further, the data generated using the OECD TG439 (in vitro skin irritation) are scattered in difference databases, and it is not easy to navigate through them. Therefore, we complied \'Reference Chemical Database System for Skin Irritation Alternative Test (RCDS-Skin Irritation)\' to allow easy, one-stop access to test chemical information. We established the systematic RCDS-Skin Irritation by collecting physiochemical properties, CAS number, human data, and in vivo (OECD TG404) data from overseas chemicals database including European Chemicals Agency (ECHA) etc., and in vitro data using Reconstructed human Epidermis (RhE) (OECD TG439). As a result, we developed the RCDS-Skin Irritation that contains information on 149 chemicals including the data we generated by performing tests using EpiDerm™ SIT, SkinEthic™ RHE and KeraSkin™ SIT. Therefore, the RCDS-Skin Irritation established based on our study will provide insight for safety assessment of chemicals and for development of alternative test methods.