As a new type of environmental pollutant, micro(nano)plastics have become a research hotspot in recent years, and their effects on the full life history of marine microalgae have not been studied. To investigate the effects of micro(nano)plastics on the growth, photosynthesis, physiological morphology and interaction of microalgae during the full life cycle, this study selected fluorescently stained polystyrene (PS) plastic microbeads as the target pollutant. By sampling and testing the growth rate, photosynthesis and physiological morphology parameters of algal species, the influence of different concentrations of PS (10, 50 and 100 mg/L) and different particle sizes (0.1, 0.5 and 1 μm) on the full life history of Skeletonema costatum (S. costatum) was investigated. The results showed that after adding PS (particle sizes of 0.5 and 1 μm), the response of S. costatum showed a dual character, while adding the same kind of microplastics (MPs) with a particle size of 0.1 μm inhibited S. costatum throughout the full life cycle. Compared with previous studies, short-term experimental data may overestimate the true ecological risks of MPs. In addition, 0.1 μm fluorescent-stained MPs obviously accumulated around the microalgae, indicating that MPs mainly adhered to the surface of algal cells and may enter the food chain in direct or indirect ways, which can cause negative effects on the aquatic ecosystem. This study supports a more accurate assessment of the true risk of MPs to marine aquatic ecosystems.

Microplastics (MPs) affect the physicochemical algal-dissolved organic matter properties, indirectly influencing the environmental behavior of contaminants including persistent organic pollutants and heavy metals. Limited research is available on the roles played by intracellular- and extracellular-dissolved organic matter (I-DOM and E-DOM) in the processes that affect the environmental behavior of contaminants. Furthermore, the effects of MPs on the production of I-DOM and E-DOM, as well as their environmental behaviors, remain uncertain. A critical issue lies in the challenge of quantitatively identifying I-DOM and E-DOM in situ. In this work, a new fluorescence ratio method was developed and applied to in situ examine the impacts of polystyrene (PS) MPs (50, 500 nm, and 5 μm) on the I-DOM and E-DOM released by Skeletonema costatum (S. costatum). The experimental results indicated that the detection limits were 0.06 mg L-1, with the respective minimum detectable proportions being 2% for both E-DOM and I-DOM. The suppressive effects of 10-50 mg L-1 of 50 and 500 nm PS MPs on the cell proliferation of S. costatum and the E-DOM secretion were most pronounced on day 6. And the rates of suppression of E-DOM secretion were 10.1%-18.2% and 4.2%-13.9%, respectively. The exposure of algal cells to 50 mg L-1 of 50 and 500 nm PS MPs led to cell rupture and the leakage of I-DOM on day 6. This suggests that the developed method in the laboratory could offer a promising approach for studying the generation of E-DOM and I-DOM in situ, as well as their environmental behaviors affected by MPs.

Skeletonema costatum, a cosmopolitan diatom primarily inhabiting coastal ecosystems, exhibits a typically close yet variable relationship with heterotrophic bacteria. The increasing temperature of surface seawater is expected to substantially affect the viability and ecological dynamics of S. costatum, potentially altering its relationship with bacteria. However, it remains unclear to what extent the elevated temperature could change these relationships. Here, the relationship between axenic S. costatum and natural seawater bacteria underwent a dramatic shift from mutualism to antagonism as the co-culture temperature increased from 20°C to 25°C. The co-occurrence network indicated significantly increased complexity of interaction between S. costatum and bacteria community after temperature elevation, especially with Flavobacteriaceae, implying their potential role in eliminating S. costatum under higher temperatures. Additionally, a Flavobacteriaceae isolate, namely MS1 identified as Tamlana genus, was isolated from the co-culture system at 25°C. MS1 had a remarkable ability to eliminate S. costatum, with the mortality rate at 25°C steadily rising from 30.2% at 48 h to 92.4% at 120 h. However, it promoted algal growth to some extent at 20°C. These results demonstrated that increased temperature promotes MS1 shifts from mutualism to antagonism with S. costatum. According to the comparative genomics analysis, changes in the lifestyle of MS1 were attributed to the increased gliding motility and attachment of MS1 under elevated temperature, enabling it to exert an algicidal effect through direct contact with alga. This investigation provided an advanced understanding of interactions between phytoplankton and bacteria in future warming oceanic ecosystems.
OBJECTIVE: Ocean warming profoundly influences the growth and metabolism of phytoplankton and bacteria, thereby significantly reshaping their interactions. Previous studies have shown that warming can change bacterial lifestyle from mutualism to antagonism with phytoplankton, but the underlying mechanism remains unclear. In this study, we found that high temperature promotes Tamlana sp. MS1 adhesion to Skeletonema costatum, leading to algal lysis through direct contact, demonstrating a transition in lifestyle from mutualism to antagonism with increasing temperature. Furthermore, the gliding motility of MS1 appears to be pivotal in mediating the transition of its lifestyle. These findings not only advance our understanding of the phytoplankton-bacteria relationship under ocean warming but also offer valuable insights for predicting the impact of warming on phytoplankton carbon sequestration.

The globally distributed harmful algal blooms (HAB) species, Heterosigma akashiwo, has been found to exhibit ichthyotoxicity. Previous studies have shown that H. akashiwo achieves a competitive edge during bloom occurrences by inhibiting the growth of a coexisting diatom, Skeletonema costatum, through allelopathy. However, the specific allelopathic mechanisms underlying the allelopathic effects of H. akashiwo on S. costatum remain unknown. To bridge this gap, our study utilized a combination of quantitative real-time PCR and metabolomics to examine the allelopathic processes of H. akashiwo on S. costatum. Our results demonstrate that the growth of S. costatum is hindered when co-cultured with H. akashiwo (initial cell concentration, 2 × 104 cell/mL). Gene expression investigation showed a substantial reduction in the mRNA levels of cytochrome b6, ribulose bisphosphate carboxylase large chain, and silicon transporter in S. costatum when grown in co-culture conditions. Furthermore, metabolic pathway analysis suggested that the allelopathic effects of H. akashiwo disrupted several vital metabolic pathways in S. costatum, including a reduction in purine and pyrimidine metabolism and an increase in fatty acid biosynthesis. Our investigation has revealed the intricate and substantial involvement of allelopathy in the formation of H. akashiwo blooms, demonstrating the complexity of the allelopathic interaction between H. akashiwo and S. costatum. These insights also contribute significantly to our understanding of the dynamics within HAB species.

Global climate changes induce substantial alterations in the marine system, including ocean acidification (OA), desalination and warming of surface seawater. Here, we examined the combined effects of OA and reduced salinity under different temperatures on the growth and photosynthesis of the diatom Skeletonema costatum. After having been acclimated to 2 CO2 concentrations (400 μatm, 1000 μatm) and 2 salinity levels (20 psu, 30 psu) at temperature levels of 10 °C and 20 °C, the diatom showed enhanced growth rate at the lowered salinity and elevated pCO2 irrespective of the temperature. The OA treatment increased the net photosynthetic rate and biogenic silica (Bsi) contents. Increasing the temperature from 10 to 20 °C raised the net photosynthetic rate by over twofold. The elevated pCO2 increased the net and gross photosynthetic rates by 20%-40% and by 16%-32%, respectively, with the higher enhancement observed at the higher levels of salinity and temperature. Our results imply that OA and desalination along with warming to the levels tested can enhance S. costatum\'s competitiveness in coastal phytoplankton communities under influence of future climate changes.

In order to improve the understanding of the environmental impacts of polymetallic nodule mining, ecotoxicological studies were conducted on the growth of model phytoplankton species Skeletonema costatum and Prorocentrum donghaiense using cobalt and nickel. This study evaluated various physiological and ecological indicators, such as cell proliferation, chlorophyll a, pigments, total protein, and antioxidant enzyme markers. The results show that the introduction of low amounts of cobalt or nickel increased the growth rate of phytoplankton. The phytoplankton benefited from low concentrations of cobalt and nickel stress. The increased protein levels and decreased activity of antioxidant enzymes considerably impacted physiological responses during the promotion of cell abundance. High concentrations of cobalt or nickel resulted in decreased light-absorbing pigments, increased photoprotective pigments, an inactive chlorophyll content, decreased total proteins, and maximal antioxidant enzyme activity in phytoplankton. Throughout the experiment, both the phytoplankton protein and enzyme activity declined with prolonged stress, and the cells underwent age-induced damage. Thus, seabed mining\'s repercussions on phytoplankton could result in both short-term growth promotion and long-term damage. These consequences depend on the impurity concentrations infiltrating the water, their duration, and the organism\'s physiological responses.

Algicidal bacteria exhibit promising potential against harmful algal blooms (HABs); however, their application has been limited due to their limited algicidal activity. This study demonstrates the enhanced algicidal activity of Alteromonas sp. FDHY-CJ bacteria against harmful Skeletonema costatum using a 5 L fermenter. Utilizing this refined framework increased the OD600 value and algal cell mortality by 6.50 and 2.88 times, respectively, compared to non-optimized culture cultivated in a flask using marine broth 2216E medium. The mechanism of action involves significant inhibition of algal photosynthetic efficiency with concurrent degradation of photosynthetic pigments. Relative to the non-optimized group, the optimized bacterial treatment led to a significant increase in H2O2 and MDA (malondialdehyde) by 19.54 and 4.22-fold, respectively, and resulted in membrane damage. The culture optimization procedure yielded effectual algicidal substances capable of considerably reducing the severity of S. costatum HABs through cell membrane disruption.

Dimethylsulphide is a dominant biogenic sulphur anti-greenhouse gas produced by marine phytoplankton. A non-axenic culture of Skeletonema costatum was studied to comprehend the effects of different growth stages and light stress on DMSP/DMS production. The intracellular DMSP concentration increased during late exponential to mid-stationary phase and attained a maximum (0.59 pg S cell-1) during the stationary phase, indicating more contribution from actively dividing smaller cells. Likewise, exposure to first light after a 12-hour dark phase caused stress, invariably leading to elevated levels of DMS (~9 fold). These observations were upheld by additional laboratory and field experiments, and a field time-series observation, which recorded higher DMS concentrations during exposure to first light after a dark cycle and during early mornings, respectively. While our study depicts the variable DMSP and DMS concentrations during different growth stages of S. costatum, it gives new information on the effect of light stress on DMS production.

The diatom Skeletonema costatum and the haptophyte Phaeocystis globosa often form blooms in the coastal waters of the South China Sea. Skeletonema costatum commonly dominates in nutrient enrichment coastal waters, whereas P. globosa starts flourishing after the diatom blooms when phosphorus (P) is limited. Therefore, P limitation was proposed to be a critical factor affecting diatom-haptophyte transition. To elucidate the tolerance to P limitation in P. globosa compared with S. costatum, the effect of P limitation on their photosystem II (PSII) performance was investigated and their photosynthesis acclimation strategies in response to P limitation were evaluated. P limitation did not affect the growth of P. globosa over 7 days but decreased it for S. costatum. Correspondingly, the PSII activity of S. costatum was significantly inhibited by P limitation. The decline in PSII activity in S. costatum under P limitation was associated with the impairment of the oxygen-evolving complex (the donor side of PSII), the hindrance of electron transport from QA - to QB (the acceptor side of PSII), and the inhibition of electron transport to photosystem I (PSI). The 100% decrease in D1 protein level of S. costatum after P limitation for 6 days and PsbO protein level after 2 days of P limitation were attributed to its enhanced photoinhibition. In contrast, P. globosa maintained its photosynthetic activity with minor impairment of the function of PSII. With accelerated PSII repair and highly increased non-photochemical quenching (NPQ), P. globosa can avoid serious PSII damage under P limitation. On the contrary, S. costatum decreased its D1 restoration under P limitation, and the maximum NPQ value in S. costatum was only one-sixth of that in P. globosa. The present work provides extensive evidence that a close interaction exists between the tolerance to P limitation and photosynthetic responses of S. costatum and P. globosa.

There are limited data available related to the sensitivity of Arctic species to environmental contaminants, and this knowledge gap creates uncertainty in environmental risk assessments (ERAs). To help address this concern, we optimized culturing conditions to allow for toxicity tests with an Arctic diatom, Nitzschia frigida. We found optimal conditions for growth were Harrison\'s medium with natural seawater at 2 °C under a continuous photoperiod of 90 μmol photons m2 s-1. We then compared the response of N. frigida with the temperate standard diatom species Skeletonema costatum. We performed concurrent and repeated exposures of the two species to three compounds (zinc, copper, and 1-methylnaphthalene). EC50 values calculated from N. frigida exposures were consistently lower than those from S. costatum tests for metals, but not 1-methylnaphthalene. Overall, we have taken the inaugural steps toward the development of a new toxicity test method using an Arctic species to inform ERAs in northern regions.