作物的内生真菌可以通过各种作用机制促进植物生长(即,提高养分吸收和养分利用效率,并产生和调节植物激素)。芸苔属包括重要的园艺作物,与内生真菌相互作用的研究很少。以前,从羽衣甘蓝根中分离出四种内生真菌(甘蓝。acephala),对他们的主人有不同的好处,包括促进植物生长,耐寒性,并诱导对病原体(油菜黄单胞菌)和害虫(油菜)的抗性。在目前的工作中,对四种不同的分离株进行了分子和形态鉴定,将它们描述为迷走神经,Setophomaterrestris,尖孢镰刀菌,和新物种Pyrenophoragallaeciana。此外,使用每种芸苔属三角形物种的代表性作物和各种体外生化测试,描述了这些真菌促进植物生长的能力。在这个意义上,使用的四种真菌促进了B.rapa的生长,B.napus,B.Nigra,B.commoea,和B.Carinata,可能是由于生长素的产生,铁载体,P溶解或纤维素酶,木聚糖酶或淀粉酶活性。最后,研究了4种内生真菌和2种病原菌(黄体细菌丝和菌核病菌)的根系定植差异和根系芥子油苷分布,在不同的时间。这样,确定了根中progoitrin的存在如何减少内生真菌和病原真菌的定植,虽然西尼格林可能水解为杀真菌产品控制了内生真菌的定植,但不是病原体。
Endophytic fungi of crops can promote plant growth through various mechanisms of action (i.e., improve nutrient uptake and nutrient use efficiency, and produce and modulate plant hormones). The genus Brassica includes important horticultural crops, which have been little studied in their interaction with endophytic fungi. Previously, four endophytic fungi were isolated from kale roots (Brassica oleracea var. acephala), with different benefits for their host, including plant growth promotion, cold tolerance, and induction of resistance to pathogens (Xanthomonas campestris) and pests (Mamestra brassicae). In the present work, the molecular and morphological identification of the four different isolates were carried out, describing them as the species Acrocalymma vagum, Setophoma terrestris, Fusarium oxysporum, and the new species Pyrenophora gallaeciana. In addition, using a representative crop of each Brassica U\'s triangle species and various in vitro biochemical tests, the ability of these fungi to promote plant growth was described. In this sense, the four fungi used promoted the growth of B. rapa, B. napus, B. nigra, B. juncea, and B. carinata, possibly due to the production of auxins, siderophores, P solubilization or cellulase, xylanase or amylase activity. Finally, the differences in root colonization between the four endophytic fungi and two pathogens (Leptosphaeria maculans and Sclerotinia sclerotiorum) and the root glucosinolate profile were studied, at different times. In this way, how the presence of progoitrin in the roots reduces their colonization by endophytic and pathogenic fungi was determined, while the possible hydrolysis of
sinigrin to fungicidal products controls the colonization of endophytic fungi, but not of pathogens.