■尽管有6%-20%的成年人患有耳鸣,没有标准的治疗方法。胎盘提取物已被用于各种治疗目的,包括听力损失。这里,我们评估了一种新型神经保护蛋白组合物(NPPC)提取物对耳鸣诱导的大鼠内侧膝状体(MGB)的电生理和分子变化的影响。
■为了通过蛋白质印迹评估蛋白质分析,将大鼠分为三组:(1)生理盐水组(腹腔注射200mg/kg生理盐水,每天两次,连续28天,(2)慢性Na-Sal组如第一组一样接受水杨酸钠,和(3)慢性治疗组(接受水杨酸200mg/kg,每天两次,持续2周,然后从第14天至第28天每天0.4mgNPPC)。在如第4组那样处理的单独的组中进行单单元记录。进行声惊吓的间隙前脉冲抑制(GPIAS)和脉冲前抑制(PPI)以确认所有组的基线耳鸣,第14天和第28天
■Westernblot分析表明,γ-氨基丁酸Aα1亚基(GABAAα1)的表达,N-甲基-d-天冬氨酸受体亚型2B(NR2B或NMDAR2B),α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体亚基GluR1(GluR1),和α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体亚基GluR2(GluR2)在Na-Sal注射后降低,而NPPC上调它们的表达。耳鸣大鼠的MGB单位显示自发放电率降低,每分钟爆裂,和一个尖峰。NPPC管理后,神经活动模式显示NPPC对耳鸣有显著的正效应。
■NPPC可以在水杨酸盐诱导的大鼠耳鸣的治疗中发挥有效作用,MGB是参与这些过程的大脑区域之一。
■NA。
UNASSIGNED: Despite 6%-20% of the adult population suffering from tinnitus, there is no standard treatment for it. Placenta extract has been used for various therapeutic purposes, including hearing loss. Here, we evaluate the effect of a novel neuroprotective protein composition (NPPC) extract on electrophysiological and molecular changes in the medial geniculate body (MGB) of tinnitus-induced rats.
UNASSIGNED: To evaluate the protein analysis by western blot, the rats were divided into three groups: (1) saline group (intraperitoneal injection of 200 mg/kg saline twice a day for 28 consecutive days, (2) chronic Na-Sal group received sodium salicylate as in the first group, and (3) chronic treatment group (received salicylate 200 mg/kg twice daily for 2 weeks, followed by 0.4 mg NPPC daily from day 14 to day 28). Single-unit recordings were performed on a separate group that was treated as in group 4. Gap-prepulse inhibition of the acoustic startle (GPIAS) and pre-pulse inhibition (PPI) was performed to confirm tinnitus in all groups at the baseline, 14th and 28th days.
UNASSIGNED: Western blot analysis showed that the expression of γ-Aminobutyric acid Aα1 subunit (GABA Aα1), N-methyl-d-aspartate receptor subtype 2B (NR2B or NMDAR2B), α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors subunit GluR1 (GluR1), and α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors subunit GluR2 (GluR2) decreased after Na-Sal injection, while NPPC upregulated their expression. MGB units in rats with tinnitus showed decreased spontaneous firing rate, burst per minute, and a spike in a burst. After NPPC administration, neural activity patterns showed a significant positive effect of NPPC on tinnitus.
UNASSIGNED: NPPC can play an effective role in the treatment of tinnitus in salicylate-induced rats, and MGB is one of the brain areas involved in these processes.
UNASSIGNED: NA.