背景:\'红阳\'猕猴桃(Actinidiachinensiscv\''红阳\')是一种优质品种,具有高产的优点,早熟,和高应力耐受性。研究证实,ShakerK+基因家族参与植物对钾(K+)的吸收和分配。
结果:从\'红阳\'猕猴桃中鉴定并分析了28个Shaker基因(A.中国cv\'红阳\')基因组。亚细胞定位结果表明,超过三分之一的AcShaker基因位于细胞膜上。系统发育分析表明,AcShaker基因分为六个亚家族(I-VI)。保守模型,基因结构,和结构域分析显示,同一亚家族的AcShaker基因具有相似的序列特征和结构。AcShaker基因的启动子顺式元件分为激素相关顺式元件和环境胁迫相关顺式元件。染色体定位和重复基因分析结果表明,AcShaker基因分布在18条染色体上,分段复制是AcShaker家族基因复制的主要模式。GO富集分析表明,AcShaker家族的离子传导途径在调节植物生长发育和逆境胁迫中起着至关重要的作用。与对照组的转录组数据相比,所有AcShaker基因在低K+胁迫下表达,三个基因(AcShaker15、AcShaker17和AcShaker22)的表达差异均极显著。qRT-PCR结果显示与转录组数据高度相关,这表明这三个差异表达基因可以调节猕猴桃植株的低钾胁迫并减少钾损伤,从而提高了对低K+应力的抵抗力。盐胁迫和对照转录组数据之间的比较表明,在盐胁迫下,AcShaker11和AcShaker18基因的表达显着不同,并且较低。表明这两个基因都可能与猕猴桃的耐盐胁迫有关。
结论:结果表明,鉴定出28个AcShaker基因,在低钾胁迫下全部表达,其中AcShaker22差异显著上调。AcShaker22基因可作为候选基因用于培育抗低钾猕猴桃新品种,为探索AcShaker基因的更多性质和功能提供参考。
BACKGROUND: \'Hongyang\' kiwifruit (Actinidia chinensis cv \'Hongyang\') is a high-quality variety of A. chinensis with the advantages of high yield, early ripening, and high stress tolerance. Studies have confirmed that the Shaker K+ genes family is involved in plant uptake and distribution of potassium (K+).
RESULTS: Twenty-eight Shaker genes were identified and analyzed from the \'Hongyang\' kiwifruit (A. chinensis cv \'Hongyang\') genome. Subcellular localization results showed that more than one-third of the AcShaker genes were on the cell membrane. Phylogenetic analysis indicated that the AcShaker genes were divided into six subfamilies (I-VI). Conservative model, gene structure, and structural domain analyses showed that AcShaker genes of the same subfamily have similar sequence features and structure. The promoter cis-elements of the AcShaker genes were classified into hormone-associated cis-elements and environmentally stress-associated cis-elements. The results of chromosomal localization and duplicated gene analysis demonstrated that AcShaker genes were distributed on 18 chromosomes, and segmental duplication was the prime mode of gene duplication in the AcShaker family. GO enrichment analysis manifested that the ion-conducting pathway of the AcShaker family plays a crucial role in regulating plant growth and development and adversity stress. Compared with the transcriptome data of the control group, all AcShaker genes were expressed under low-K+stress, and the expression differences of three genes (AcShaker15, AcShaker17, and AcShaker22) were highly significant. The qRT-PCR results showed a high correlation with the transcriptome data, which indicated that these three differentially expressed genes could regulate low-K+ stress and reduce K+ damage in kiwifruit plants, thus improving the resistance to low-K+ stress. Comparison between the salt stress and control transcriptomic data revealed that the expression of AcShaker11 and AcShaker18 genes was significantly different and lower under salt stress, indicating that both genes could be involved in salt stress resistance in kiwifruit.
CONCLUSIONS: The results showed that 28 AcShaker genes were identified and all expressed under low K+ stress, among which AcShaker22 was differentially and significantly upregulated. The AcShaker22 gene can be used as a candidate gene to cultivate new varieties of kiwifruit resistant to low K+ and provide a reference for exploring more properties and functions of the AcShaker genes.