■先前的研究表明,MSC可以减轻急性胰腺炎(AP)期间胰腺的损伤。然而,与AP相关的急性死亡率更常见的是远端器官持续衰竭的结果,而不是局部损坏,特别是在重症急性胰腺炎(SAP),MSC的作用可能因其来源而异。
■通过胆管逆行注射5%牛磺胆酸钠溶液,在8周C57BL/6J雄性小鼠中诱导SAP模型。将SAP小鼠分为SAP组,UC-MSCs组,和BMSCs组,用盐水治疗,1×106UC-MSCs,和1×106个BMSCs,通过尾静脉.治疗后,血清标记物,炎症,对胰腺进行形态学评估,肾脏,肺,和心。
■MSCs输注改善了SAP小鼠的全身炎症反应。在MSCs处理的SAP小鼠中,胰腺局部组织损伤和炎症反应减轻。但更重要的是,肾和肺损伤均显著缓解,IL-6、MCP-1、IL-1β等促炎因子水平,和肾脏中的TNF-α,肺和心脏急剧下降。在起源方面,与BMSCs相比,UC-MSCs表现出更好的疗效。此外,与正常对照小鼠相比,UC-MSCs显示较早的外观,更高的分布密度,在受伤组织中存在的持续时间更长。
这项研究提供了令人信服的证据,支持MSCs在SAP治疗中的治疗潜力,特别是它们减轻多器官衰竭的能力。我们的结果还表明,UC-MSCs在SAP治疗中可能比BMSCs具有更大的优势。
UNASSIGNED: Previous studies showed that MSCs could mitigate damage in the pancreas during acute pancreatitis (AP). However, acute mortality associated with AP was more often a result of persistent failure of remote organs, rather than local damage, especially in severe acute pancreatitis (SAP), and the effect of MSCs may vary depending on their origin.
UNASSIGNED: An SAP model was induced in 8-week C57BL/6 J male mice by retrograde injection of 5 % sodium taurocholate solution through the bile duct. SAP mice were divided into the SAP group, UC-MSCs group, and BMSCs group, which were treated with saline, 1 × 106 UC-MSCs, and 1 × 106 BMSCs respectively, through the tail vein. After treatment, serum markers, inflammation, and morphology were assessed in the pancreas, kidneys, lungs, and hearts.
UNASSIGNED: MSCs infusion ameliorated the systemic inflammatory response in SAP mice. In the MSCs-treated SAP mice, local tissue injury and inflammation response in the pancreas were alleviated. But more importantly, the renal and lung injury were all significantly and drastically mitigated, and the levels of pro-inflammatory factors such as IL-6, MCP-1, IL-1β, and TNF-α in the kidney, lung and heart were sharply decreased. In terms of origin, UC-MSCs exhibited superior efficacy compared with BMSCs. Furthermore, compared to the normal control mice, UC-MSCs showed an earlier appearance, higher distribution densities, and longer duration of presence in the injured tissue.
UNASSIGNED: This study provides compelling evidence supporting the therapeutic potential of MSCs in SAP treatment and particularly their ability to mitigate multi-organ failure. Our results also suggested that UC-MSCs may offer greater advantages over BMSCs in SAP therapy.