与糖尿病相关的最常见的并发症之一是脑内的细胞凋亡,其可导致认知障碍。运动被认为是通过知之甚少的机制来降低细胞死亡的严重程度和程度的最佳非药理学方法。这项研究的目的是调查自愿复杂和定期车轮运行对8-氧鸟嘌呤DNA糖基化酶(OGG1)水平的影响,信号素3B(sema3B),过氧化氢(H2O2),糖尿病大鼠海马细胞凋亡。
48只Wistar雄性大鼠随机分为6组:健康对照组(C),糖尿病控制(D),常规车轮运行+糖尿病(RWD),复杂的车轮运行+糖尿病(CWD),健康的常规车轮运行(RW),和健康的复杂车轮运行(CW)。通过腹腔注射链脲佐菌素(STZ)产生糖尿病大鼠模型。该方案包括在常规和复杂的车轮运行设备上进行为期4周的自愿运行训练方案。在最后一次训练后48小时处死大鼠。为了测量海马体内的蛋白质浓度,已经使用了ELISA。使用单因素方差分析比较各组。
两组之间的OGG1蛋白水平没有显著差异。D组的H2O2水平明显高于C组(p=0.002),而RWD和CWD组比D组低得多(分别为p=0.002和p=0.003)。在D组,细胞凋亡和Sema3B的水平(分别为p=.001和p=.007)显著高于C,RWD(分别为p=.001,p=.0001),和CWD组(分别为p=.001,p=.006)。然而,RWD组和CWD组之间无显著差异.
Sema3B水平的提高,通过两种类型的自愿车轮运行方案,可以显着恢复与糖尿病相关的海马内的H2O2和凋亡。
One of the most frequent complications associated with diabetes mellitus is apoptosis within the brain which can lead to cognitive disorders. Exercise is considered the best non-pharmacological approach to reduce the severity and extent of cell death through poorly-understood mechanisms. The aim of this study was to investigate the effects of voluntary complex and regular wheel running on the levels of 8-oxoguanine DNA glycosylase (OGG1 ), semaphorin 3B (sema3B), hydrogen peroxide (H2 O2 ), and apoptosis in the hippocampus of diabetic rats.
48 Wistar male rats were randomly divided into 6 groups: healthy control (C), diabetes control (D), regular wheel running + diabetes (RWD), complex wheel running + diabetes (CWD), healthy regular wheel running (RW), and healthy complex wheel running (CW). The diabetic rat model was produced by intraperitoneal injection of streptozotocin (STZ). The protocol encompassed a 4-week voluntary running training regimen on regular and complex wheel running apparatus. The rats were sacrificed 48 hr after the last training session. To measure the protein concentrations within the hippocampus, ELISA has been utilized. One-way ANOVA was used to compare the groups.
There were no significant differences in OGG1 protein levels between the groups. H2 O2 level in the D group was significantly higher than the C group (p = .002), while this in RWD and CWD groups was considerably lower than the D group (p = .002 and p = .003, respectively). In the D group, the levels of apoptosis and Sema3B were significantly (p = .001 and p = .007, respectively) higher than C, RWD (p = .001, p = .0001, respectively), and CWD groups (p = .001, p = .006, respectively). Nevertheless, there were not any significant differences between RWD and CWD groups.
The increased levels of Sema3B, H2O2, and apoptosis within the hippocampus associated with diabetes could be noticeably restored by both types of voluntary wheel running protocols.