■评估LaurusNobilis滴眼液对乳兔亚硒酸盐引起的白内障的保护作用。
■将15只没有眼部炎症迹象的雄性白化乳兔随机分为三组:对照组(A组),亚硒酸钠组(B组)和亚硒酸钠加诺比利斯组(C组)。通过亚硒酸盐处理,实验诱导白内障形成,然后分级。在晶状体中定义了氧化应激的等级,测量丙二醛的浓度,α-生育酚,氧化型谷胱甘肽,抗坏血酸和过氧化氢,以及血液样本中α-生育酚和丙二醛的水平。
■GSSG的平均晶状体浓度,B组的H2O2和MDA水平明显高于C组和对照组。B组的抗坏血酸和α-生育酚浓度低于C组和A组。MDA水平在B组高于C组和A组。B组的平均α-生育酚水平显著低于A组和C组。炎症被抑制,脂质过氧化反应显著降低。Laurusnobilis叶提取物是抗氧化剂成分的良好来源,可以与亚硒酸钠诱导的乳兔白内障形成对比。
UNASSIGNED: To evaluate the protective role of Laurus Nobilis eye drops on
selenite-induced cataracts in suckling rabbits.
UNASSIGNED: Fifteen male albino suckling rabbits with no signs of ocular inflammation were randomly assigned to three groups: controls (Group A), sodium-
selenite group (Group B) and sodium-
selenite plus Laurus Nobilis group (Group C). By
selenite treatment, cataract formation was experimentally induced and then graded. The grade of oxidative stress was defined in the lens, measuring the concentration of malondialdehyde, alpha-tocopherol, oxidized glutathione, ascorbic acid and hydrogen peroxide, and in blood samples as levels of alpha-tocopherol and malondialdehyde.
UNASSIGNED: Mean lens concentrations of GSSG, H2O2, and MDA levels in group B were significantly higher than in both group C and control. Ascorbic acid and alpha-tocopherol concentrations were lower in group B than in both group C and A. As plasma oxidative status markers, the level of MDA was higher in group B respected group C and A. The mean alpha-tocopherol levels in group B were significantly lower than in both group A and group C.
UNASSIGNED: In animals treated with Laurus Nobilis-based eye drops, inflammation was inhibited, and lipid peroxidation was significantly reduced. Laurus nobilis leaves extract represents a good source of antioxidant components that may contrast sodium
selenite-induced cataractogenesis in suckling rabbits.