由于高增长率,突出的非生物胁迫耐受性,和丰富的增值物质,RoscoffensisChrysotila,属于齿科门,可以被认为是生物活性化合物工业开发的通用资源。然而,直到最近,C.roscoffensis的应用潜力才引起人们的关注,对该物种的生物学特性的了解仍然很少。例如,克罗夫斯科对抗生素的敏感性,这对于验证异养能力至关重要,目前尚无法建立高效的基因操纵系统。旨在为未来的开发提供基础信息,本研究测试了C.roscoffensis对9种抗生素的敏感性。结果表明,克罗夫特对氨苄青霉素表现出相对较高的抗性,卡那霉素,链霉素,庆大霉素,和遗传霉素,虽然对博来霉素敏感,潮霉素B,巴龙霉素,和氯霉素.使用前五种抗生素,初步建立了细菌去除策略。最后,基于包括固体平板在内的多策略方法,16SrDNA扩增,和核酸染色。该报告可以为开发最佳选择标记提供有价值的信息,这对于在C.roscoffensis中进行更广泛的转基因研究是有意义的。此外,我们的研究还为建立C.roscoffensis的异养/混养栽培模式铺平了道路。
Due to high growth rate, outstanding abiotic stress tolerance, and rich value-added substances, Chrysotila roscoffensis, belonging to the phylum of Haptophyta, can be considered as a versatile resource for industrial exploitation of bioactive compounds. However, the application potential of C. roscoffensis has drawn attention until just recently, and the understanding related to the biological properties of this species is still scarce. For example, the sensitivities of C. roscoffensis to antibiotics, which is essential for the verification of heterotrophic capacity and the establishment of efficient genetic manipulation system is still unavailable. Aiming to provide fundamental information for future exploitation, the sensitivities of C. roscoffensis to nine types of antibiotics were tested in this study. The results demonstrated that C. roscoffensis exhibited relatively high resistances to ampicillin, kanamycin, streptomycin, gentamicin, and geneticin, while was sensitive to bleomycin, hygromycin B, paromomycin, and chloramphenicol. Using the former five types of antibiotics, a bacteria removal strategy was established tentatively. Finally, the axenicity of treated C. roscoffensis was confirmed based on a multi-strategy method including solid plate, 16S rDNA amplification, and nuclear acid staining. This report can provide valuable information for the development of optimal selection markers, which are meaningful for more extensive transgenic studies in C. roscoffensis. Moreover, our study also paves the way for the establishment of heterotrophic/mixotrophic cultivation modes of C. roscoffensis.