目的:先天性免疫反应,特别是多形核中性粒细胞(PMN)的反应,对塑造慢性炎症的结局至关重要,纤维化,或生物材料植入后的骨整合。种植体周围炎或黏膜炎,与牙科植入物有关的炎症状况,对植入成功构成重大威胁。我们使用鼠模型开发了一种单细胞分析方法来评估对植入材料的免疫反应,为潜在的牙科植入物提供实用的筛选工具。
方法:我们进行了生物信息学分析,并通过将两个钛植入物插入上颌区域来建立种植体周围炎症模型,检查免疫反应。
结果:生物信息学分析显示,钛植入物引发了宿主免疫反应,主要由PMN介导。在体内实验中,我们观察到PMN介导的快速反应,到第3天,植入物周围和植入物表面的浸润增加。值得注意的是,PMN对植入物的附着持续7天,类似于在人类植入物介导的炎症中看到的免疫谱。
结论:我们的研究结果表明,短寿命PMN对钛植入物的持续附着可以作为植入物周围炎症的指标或特征。因此,在单细胞水平上分析牙龈组织可能是评估候选牙种植体生物相容性的有用工具。
OBJECTIVE: The innate immune response, particularly the reaction of polymorphonuclear neutrophils (PMNs), is crucial in shaping the outcomes of chronic inflammation, fibrosis, or osseointegration following biomaterial implantation. Peri-implantitis or peri-mucositis, inflammatory conditions linked to dental implants, pose a significant threat to implant success. We developed a single-cell analysis approach using a murine model to assess the immune response to implant materials, offering a practical screening tool for potential dental implants.
METHODS: We performed bioinformatics analysis and established a peri-implant inflammation model by inserting two titanium implants into the maxillary region, to examine the immune response.
RESULTS: Bioinformatics analysis revealed that titanium implants triggered a host immune response, primarily mediated by PMNs. In the in vivo experiments, we observed a rapid PMN-mediated response, with increased infiltration around the implants and on the implant surface by day 3. Remarkably, PMN attachment to the implants persisted for 7 days, resembling the immune profiles seen in human implant-mediated inflammation.
CONCLUSIONS: Our findings indicate that persistent attachment of the short-living PMNs to titanium implants can serve as an indicator or traits of peri-implant inflammation. Therefore, analyzing gingival tissue at the single-cell level could be a useful tool for evaluating the biocompatibility of candidate dental implants.