目的:虎耳草科(Saxifragasinomontana)是青藏高原及其两侧山区广泛分布的高山物种。我们开发了一组表达序列标签-简单序列重复(EST-SSR)标记,以研究该物种的遗传多样性和进化史。
结果:我们最初基于S.sinomontana的转录组数据设计了50个EST-SSR标记。50个基因座中有19个(38%)成功扩增,其中13个是多态的。对来自四个种群的71个人进行了测试。每个基因座检测到3到18个等位基因,观察到的杂合度和预期的杂合度分别为0.2817至0.9155和0.2585至0.8495。此外,三个同属物种的所有13个基因座都成功进行了交叉扩增,S、Tangutica,S、海伦士,和美国。
结论:这些EST-SSR标记将有助于研究S.sinomontana的遗传多样性和解开相关物种的系统发育关系。
OBJECTIVE: Saxifraga sinomontana (Saxifragaceae) is a widespread alpine species in the Qinghai-Tibetan Plateau and its flanking mountains. We developed a set of expressed sequence tag-simple sequence repeat (EST-SSR) markers to investigate the genetic diversity and evolutionary history of the species.
RESULTS: We initially designed 50 EST-SSR markers based on transcriptome data of S. sinomontana. Nineteen of 50 loci (38%) were successfully amplified, 13 of which were polymorphic. These were tested on 71 individuals from four populations. Three to 18 alleles per locus were detected, and the levels of observed and expected heterozygosity ranged from 0.2817 to 0.9155 and 0.2585 to 0.8495, respectively. In addition, cross-amplification was successful for all 13 loci in three congeneric species, S. tangutica, S. heleonastes, and S. congestiflora.
CONCLUSIONS: These EST-SSR markers will be useful for studying the genetic diversity of S. sinomontana and disentangling the phylogenetic relationships of related species.