肠炎沙门氏菌(S.肠炎)是一种宿主范围的病原体,可以感染动物和人类。禽类和禽类产品是肠炎沙门氏菌的主要载体,可以通过食物链传播给人类。为了根除禽类养殖场中肠炎沙门氏菌的流行,有必要开发针对病原体的新型疫苗。在这项研究中,我们构建了两种候选疫苗,CZ14-1ΔspiCΔnmpC和CZ14-1ΔspiCΔrfal,并评估其保护功效。两种突变菌株的毒力都比亲本菌株低得多,由三天大的无特定病原体(SPF)白角和Hyline白鸡的50%致死剂量(LD50)确定。用突变体候选物免疫诱导高度特异性的体液免疫应答和细胞因子IFN-γ的表达,IL-1β,IL-6此外,发现突变菌株在感染后持续存在近三周。用CZ14-1ΔspiCΔnmpC和CZ14-1ΔspiCΔrfaL免疫的鸡的存活率分别达到80%和75%,分别,在与父母菌株挑战后。总的来说,这些结果表明,这两个突变株可以作为减毒活疫苗开发。
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a host-ranged pathogen that can infect both animals and humans. Poultry and poultry products are the main carriers of S. Enteritidis, which can be transmitted to humans through the food chain. To eradicate the prevalence of S. Enteritidis in poultry farms, it is necessary to develop novel vaccines against the pathogen. In this study, we constructed two vaccine candidates, CZ14-1∆spiC∆nmpC and CZ14-1∆spiC∆rfaL, and evaluated their protective efficacy. Both mutant strains were much less virulent than the parental strain, as determined by the 50% lethal dose (LD50) for three-day-old specific-pathogen free (SPF) White Leghorns and Hyline White chickens. Immunization with the mutant candidates induced highly specific humoral immune responses and expression of cytokines IFN-γ, IL-1β, and IL-6. In addition, the mutant strains were found to be persistent for almost three weeks post-infection. The survival percentages of chickens immunized with CZ14-1∆spiC∆nmpC and CZ14-1∆spiC∆rfaL reached 80% and 75%, respectively, after challenge with the parental strain. Overall, these results demonstrate that the two mutant strains can be developed as live attenuated vaccines.