本研究旨在使用口服灌胃攻击模型评估火鸡中沙门氏菌雷丁(SR)的命运和传播。一百二十八周龄的商业火鸡母鸡从商业生产转移到研究机构。抵达后,恩诺沙星的组合,10mg/kg,和氟苯尼考,20mg/kg,在混合放置在新鲜的松屑上之前,依次口服给药。在第4天和第7天,通过口服管饲法对火鸡进行108cfuSR攻击。子集在d14(n=40)进行模拟商业处理,21(n=40)和28(n=32)安置后(对应于10、11和12周龄)。倾斜接头,皮肤,气管,作物,肺,肝+脾(LS),并对盲肠进行无菌采样和培养,以进行沙门氏菌回收和血清分型。接种后14d(PI)无法从窒息关节恢复SR。然而,在14dPI时,SR的回收率为:皮肤80%;作物75%;LS67.5%;肺60%;和盲肠57.5%。(P<0.01)。有趣的是,从气管观察到SR的最低恢复(40%)。在21dPI时,在盲肠(87.5%)和作物(67.5%)中观察到最高的SR阳性样品率。由28dPI,SR仅从盲肠(75%)、作物(43.8%)、肺(34.4%)和LS(21.9%)中回收。这项研究的结果证实,SR是火鸡行业的一个新问题,减少沙门氏菌等食源性病原体的即时措施应针对供应链的所有部分和消费者关于食品安全的教育。
This study aimed to evaluate the fate and dissemination of Salmonella Reading (SR) in turkeys using an oral gavage challenge model. One hundred twenty-eight-week-old commercial turkey hens were moved from commercial production to research facilities. Upon arrival, a combination of enrofloxacin, 10 mg/kg, and florfenicol, 20 mg/kg, were orally administered sequentially before comingled placement on fresh pine shavings. Turkeys were challenged with 108 cfu SR by oral gavage on d 4 and 7 postplacement. Subsets were subjected to simulated commercial processing on d 14 (n = 40), 21 (n = 40) and 28 (n = 32) postplacement (corresponding to 10, 11, and 12 wk of age). Stifle joint, skin, trachea, crop, lung, liver + spleen (LS), and ceca were aseptically sampled and cultured for Salmonella recovery and serotyping. SR could not be recovered from stifle joint 14 d post inoculation (PI). However, at 14 d PI, recovery of SR were: Skin 80%; crop 75%; LS 67.5%; lungs 60%; and ceca 57.5%. (P < 0.01). Interestingly, the lowest recovery of SR was observed from trachea (40%). At 21 d PI, the highest rate of positive samples to SR were observed in ceca (87.5%) and crop (67.5%). By 28 d PI, SR was only recovered from ceca (75%); crop (43.8%); lung (34.4%); and LS (21.9%). The results of this study confirms that SR is an emerging problem for the turkey industry and immediate measurements to reduce foodborne pathogens such as Salmonella should target all parts of the supply chain and consumer education about food safety.