微生物固定化技术被认为是一种有效的铬(Cr)污染的生物修复方法。然而,目前尚不清楚哪种菌株对Cr污染的水和土壤的修复更有利。因此,以玉米秸秆生物炭为载体,制备了固定内生菌沙雷氏菌的材料。Y-13(BSR1),线虫沙雷菌(BSR2),溶血芽孢杆菌。菌株SePC-36(BLB1),芒硝芽孢杆菌菌株WK63(BLB2)和商业细菌ShewanellaoneidensisMR-1(BSW)。结果表明,与BSW相比,内生菌负载的生物炭(尤其是BSR1)在修复水和土壤中的Cr污染方面更有效。内生菌负载生物炭降低了土壤病原菌的丰度,增加了有益植物内生菌的数量,降低土壤Cr(VI)浓度,提高土壤肥力,降低了植株Cr浓度,提高了生菜产量。冗余分析(RDA)和结构方程模型(PLS-PM)表明土壤微生物与土壤Cr(VI)密切相关,植物鲜重和土壤有机质,而内生菌负载的生物炭通过改变植物微生物直接影响植物细胞运动途径。这项研究代表了对内生菌负载生物炭作为Cr污染修复策略的功效的开创性研究。
Microbial immobilization technology is considered an efficient bioremediation method for chromium (Cr) pollution. However, it is currently unclear which strain is more beneficial for the remediation of Cr-contaminated water and soil. Therefore, corn straw biochar was used as a carrier to prepare materials for fixing the endophytes Serratia sp. Y-13 (BSR1), Serratia nematodiphila (BSR2), Lysinibacillus sp. strain SePC-36 (BLB1), Lysinibacillus mangiferihumi strain WK63 (BLB2) and the commercial bacteria Shewanella oneidensis MR-1 (BSW). The results demonstrated that, compared with BSW, endophyte-loaded biochar (especially BSR1) was more effective at remediating Cr pollution in water and soil. Endophyte-loaded biochar reduced the abundance of soil pathogenic bacteria, enhanced the number of beneficial plant endophytes, reduced the soil Cr(VI) concentration, improved soil fertility, reduced the plant Cr concentration and improved the yield of lettuce. Redundancy analysis (RDA) and structural equation modelling (PLS-PM) suggested that soil microbes are closely related to soil Cr(VI), plant fresh weight and soil organic matter, whereas endophyte-loaded biochar directly influences plant cell motility pathways by altering plant microbes. This study represents a pioneering investigation into the efficacy of endophyte-loaded biochar as a remediation strategy for Cr pollution.