■本研究旨在鉴定与男性强直性脊柱炎(AS)相关的关键基因,并分析免疫细胞浸润在该疾病病理过程中的作用。
■AS数据集从基因表达综合(GEO)公共数据库下载,提取男性健康对照组(M_HC)和男性AS患者(M_AS)的数据。R软件用于鉴定差异表达基因(DEGs)。进行DEGs的功能和途径富集分析。构建了蛋白质-蛋白质相互作用(PPI)网络,并筛选出hub基因。所有表达谱数据采用加权相关网络分析(WGCNA)筛选出hub基因,然后与PPI网络中的hub基因相交以获得关键基因。最后,用CIBERSORT评估两组样本中免疫细胞浸润的差异,并分析了关键基因与浸润免疫细胞的相关性。
■共获得689个DEG,其中395个基因上调,294个基因下调。功能和途径富集分析表明,DEGs主要富集在与免疫应答相关的途径中。根据PPI分析,选择了五个得分较高的集群。通过WGCNA,获得14个基因模块。选取相关度最高的绿色模块,与之前获得的簇相交,得到3个关键基因,RAB5C,SYNJ1和RNF19B。免疫浸润剖析发明,单核细胞和γδT细胞可能介入了AS的进程。此外,RAB5C,SYNJ1和RNF19B都与单核细胞和巨噬细胞水平升高有关。
■RAB5C,SYNJ1和RNF19B是在M_AS中表达的关键DEGs,可能通过调节免疫细胞功能在疾病的发生和发展中发挥作用。
UNASSIGNED: This study aimed to identify the key genes related to male ankylosing spondylitis (AS) and to analyze the role of immune cell infiltration in the pathological process of this disease.
UNASSIGNED: The AS dataset was downloaded from the Gene Expression Omnibus (GEO) public database, and the data of male healthy controls (M_HC) and male AS patients (M_AS) were extracted. R software was used to identify differentially expressed genes (DEGs). Functional and pathway enrichment analysis of the DEGs was performed. A protein-protein interaction (PPI) network was constructed, and the hub genes were screened out. All expression profile data were analyzed by weighted correlation network analysis (WGCNA) to screen out the hub genes, which were then intersected with the hub genes from the PPI network to obtain the key genes. Finally, the difference in immune cell infiltration in the two sets of samples was evaluated with CIBERSORT, and the correlation between the key genes and infiltrating immune cells was analyzed.
UNASSIGNED: A total of 689 DEGs were obtained, of which 395 genes were up-regulated and 294 genes were down-regulated. Functional and pathway enrichment analysis showed that DEGs were mainly enriched in pathways related to immune response. Based on the PPI analysis, five clusters with high scores were selected. Through WGCNA, 14 gene modules were obtained. The green module with the highest correlation was selected and intersected with the cluster previously obtained to obtain three key genes, RAB5C,
SYNJ1, and RNF19B. Immune infiltration analysis found that monocytes and gamma delta T cells may be involved in the process of AS. Also, RAB5C,
SYNJ1, and RNF19B are all related to increased levels of monocytes and macrophages.
UNASSIGNED: RAB5C,
SYNJ1, and RNF19B are key DEGs expressed in M_AS and may play a role in the disease\'s occurrence and development through regulating immune cell functions.