背景:肺炎球菌菌血症是全球发病率和死亡率的主要因素。传统的基于文化的方法缺乏敏感性并且耗时。本研究旨在评估两种培养无关的检测方法的有效性。MALDI-TOF-MSSepsityper®模块和标准F®肺炎链球菌的侧流免疫层析试验(LFICT),直接从阳性血培养(BC)瓶。
方法:一项前瞻性研究于2021年12月至2022年7月进行。对于肺炎链球菌的所有BC阳性,实施双离心方案。随后使用两种技术处理所得的粒料。
结果:LFCT表现出卓越的性能,具有100%的灵敏度和特异性,性能优于MALDI-TOF-MSSepsityper®模块,达到85.2%的灵敏度和100%的特异性。然而,这些检测方法的结合提供了一种稳健而全面的诊断方法.
结论:两种技术的同时使用提供了一种有希望的替代方法,可以直接从BC样品中整合到常规实践中。
BACKGROUND: Pneumococcal bacteraemia is a major contributor to global morbidity and mortality. Traditional culture-based methods lack sensitivity and are time-consuming. This study aimed to assess the effectiveness of two culture-independent assays, the MALDI-TOF-MS Sepsityper® module and the lateral flow inmunochromatography test (LFICT) with the Standard F® Streptococcus pneumoniae, directly from positive blood culture (BC) bottles.
METHODS: A prospective study was conducted from December 2021 to July 2022. For all BC positives for S. pneumoniae a double centrifugation protocol was implemented. The resulting pellet was subsequently processed using both techniques.
RESULTS: The LFICT showed exceptional performance with 100% sensitivity and specificity, outperforming the MALDI-TOF-MS Sepsityper® module, which achieved 85.2% sensitivity and 100% specificity. Nevertheless, the combination of these assays offers a robust and comprehensive approach to diagnosis.
CONCLUSIONS: The simultaneous use of both techniques offers a promising alternative that can be integrated into routine practices directly from BC samples.