系统毒理学分析(STA)是使用适当的分析方法来检测和鉴定生物样品中尽可能多的潜在毒理学相关化合物的过程。STA是法医毒理学日常工作的重要组成部分,STA的几种方法经常被独立发表和审查。然而,涉及的许多药物和其他物质,以及新的不断涌现,可能对STA构成重大挑战,这通常需要一种并行使用多种分析方法的策略。尽管在法医毒理学中具有相关性,但此类策略的发布和评估频率较低。这篇小型综述简要总结了STA在法医毒理学中的常用方法,包括气相色谱-质谱(GC-MS)和液相色谱-MS(LC-MS)方法,并强调了他们的一些潜在陷阱。第二,它概述了先前报告的进行STA的策略,包括作者实验室中应用的STA策略的介绍。这涉及通过LC-高分辨率MS进行广泛的药物筛选,通过使用LC串联MS的靶向筛选和定量来支持,顶空(HS)-GC-MS,HS-GC-火焰电离检测器和其他互补方法。STA战略旨在涵盖尽可能多的潜在相关药物,并寻求减少法医案件工作中出现的潜在陷阱。审查强调,即使有全面的STA战略,也不是在所有情况下都能识别出每种物质。
Systematic toxicological analysis (
STA) is the process of using an adequate analytical methodology to detect and identify as many potentially toxicologically relevant compounds as possible in biological samples.
STA is an important part of everyday routine work within forensic toxicology, and several methods for
STA have frequently been published and reviewed independently. However, the many drugs and other substances involved, as well as the constant emergence of new ones, may pose a major challenge in STA, which often demands a strategy involving multiple analytical methods in parallel. Such strategies have been published and evaluated less frequently despite their relevance in forensic toxicology. This mini-review briefly summarizes commonly applied methods for
STA in forensic toxicology, including gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-MS (LC-MS) methods, and highlights some of their potential pitfalls. Second, it provides an overview of previously reported strategies to conduct STA, including a presentation of the
STA strategy applied in the authors\' laboratory. This involves broad drug screening by LC-high-resolution MS, supported by targeted screening and quantification using LC-tandem MS, headspace (HS)-GC-MS, HS-GC-flame ionization detector and other complementary methods. The STA strategy aims to cover as many potentially relevant drugs as possible and seeks to reduce potential pitfalls arising in forensic casework. The review underlines that not every substance can be identified in all circumstances even with a comprehensive STA strategy.