■滑膜肉瘤(SS)是神秘的软组织肿瘤,它们还没有确定的起源细胞。根据患者的年龄和表现部位,SS具有多种鉴别诊断。除非通过逆转录聚合酶链反应(RT-PCR)或荧光原位杂交(FISH)鉴定出特定的融合SS18:SSX,否则这将使诊断变得麻烦。在资源匮乏的环境中,免疫组织化学是帮助缩小差异并帮助诊断该肿瘤的有用工具。这项研究开始评估可能的候选免疫组织化学标记物在识别SS中的实用性。
■40例SS,由鱼类证明包括在内。构建了组织微阵列(TMA),和免疫组织化学使用抗体-TLE1(OTI1F5),β-连环蛋白(14),INI1(MRQ-27),CK7(OV-TL),CK19(多克隆),SS18(多克隆),calponin(CALP),和claudin1(多克隆)。分析表达以达到敏感性和特异性。
■TLE1的敏感性为92.5%,特异性为100%;β-Catenin的敏感性为17.5%,特异性为100%;Calponin的敏感性为97.5%,特异性为81.25%;SS18的敏感性为95%,特异性为100%;INI1的敏感性为95%,特异性为100%;CK7的敏感性为9.85%,特异性为819%,特异性为9.85%;
■研究表明,TLE1和SS18是滑膜肉瘤的可靠诊断标志物,灵敏度分别为92%和95%,分别。INI1可用于区分SS与非上皮样和非横纹肌样差异。钙蛋白的表达有助于区分低分化的SS及其模拟物。CK7是比CK19更好的标记,可以在初始筛选组中用作EMA的替代品。claudin1的使用仅限于描绘上皮成分。β-连环蛋白敏感性差,限制其在SS中的效用。
UNASSIGNED: Synovial sarcomas (SS) are enigmatic soft tissue tumors, which are yet to have a defined cell of origin. SS have a variety of differential diagnosis depending upon the age of the patient and the site of presentation. This makes diagnosis cumbersome unless the specific fusion
SS18:SSX is identified by reverse transcription-polymerase chain reaction (RT-PCR) or fluorescence in situ hybridization (FISH). Immunohistochemistry is a useful tool in resource-poor settings in helping to narrow the differentials and help diagnose this tumor. This study set about assessing possible candidate immunohistochemical markers in their utility to recognize SS.
UNASSIGNED: Forty cases of SS, proven by FISH were included. A tissue microarray (TMA) was constructed, and immunohistochemistry was done using antibodies - TLE1 (OTI1F5), β-catenin (14), INI1 (MRQ-27), CK7 (OV-TL), CK19 (polyclonal),
SS18 (polyclonal), calponin (CALP), and claudin1 (Polyclonal). The expression was analyzed to arrive at sensitivity and specificity.
UNASSIGNED: TLE1 had a sensitivity of 92.5% and a specificity of 100%; β-Catenin had a sensitivity of 17.5% and specificity of 100%; Calponin had a sensitivity of 97.5% and a specificity of 81.25%;
SS18 had a sensitivity of 95% and specificity of 100%; INI1 had a sensitivity of 95% and specificity of 100%; CK7 had a sensitivity of 90% and specificity of 87.5%; CK19 had a sensitivity of 90% and a specificity of 59.38%; and Claudin had a sensitivity of 85% and a specificity of 78.12%.
UNASSIGNED: The study showed both TLE1 and
SS18 are robust diagnostic markers of synovial sarcoma with a sensitivity of 92% and 95%, respectively. INI1 can be used to discriminate SS from nonepithelioid and nonrhabdoid differentials. Calponin expression is helpful to differentiate poorly differentiated SS from its mimics. CK7 is a better marker than CK19 and can be used as a replacement for EMA in the initial screening panel. The use of claudin1 was restricted to delineating the epithelial component. β-Catenin had poor sensitivity, restricting its utility in SS.