精子相关抗原5(SPAG5)是一种有丝分裂纺锤体蛋白,可调节姐妹染色单体分离成子细胞。最近的研究发现它在各种癌症中过度表达,提示其致癌特性和功能。然而,对SPAG5关于其诊断的全面分析,预后,缺乏不同癌症类型的免疫相关效应。在这项研究中,我们采用生物信息学方法,整合多个公共数据库,探索SPAG5的潜在致癌作用.我们分析了它的表达,预后,相关化学品,丰富的途径,免疫浸润,及其对不同肿瘤遗传改变的影响。结果显示SPAG5在大多数癌症中高表达,并且与患者预后不良显著相关。此外,SPAG5表达在15种不同类型的癌症中显示出早期癌症诊断的潜力。在肿瘤免疫方面,SPAG5的高表达与免疫抑制性肿瘤微环境和免疫治疗疗效指标相关.SPAG5表达与大多数免疫细胞浸润呈负相关,但与Th2细胞和MDSC细胞呈显着正相关。多色荧光免疫组织化学显示SPAG5激活肿瘤内的免疫细胞群,表明其在肿瘤微环境中的重要作用。富集分析表明SPAG5相关基因主要参与细胞周期,细胞衰老,P53信号通路,和FoxO信号通路。此外,我们证实了SPAG5在癌细胞中的高表达,并观察到其敲低可上调p53蛋白的表达。总之,SPAG5具有诊断价值,预后,和多种癌症中的免疫生物标志物,可能为肿瘤免疫治疗提供新的靶点。
Sperm-associated antigen 5 (
SPAG5) is a mitotic spindle protein that regulates the separation of sister chromatids into daughter cells. Recent studies have discovered its overexpression in various cancers, suggesting its oncogenic characteristics and functions. However, a comprehensive analysis of
SPAG5 regarding its diagnostic, prognostic, and immune-related effects across different cancer types is lacking. In this study, we employed bioinformatics methods and integrated multiple public databases to explore the potential oncogenic role of SPAG5. We analyzed its expression, prognosis, related chemicals, enriched pathways, immune infiltration, and its impact on different tumor genetic alterations. The results revealed that
SPAG5 is highly expressed in most cancers and significantly correlates with poor patient prognosis. Additionally,
SPAG5 expression showed potential for early cancer diagnosis in 15 different cancer types. In terms of tumor immunity, high expression of SPAG5 was associated with an immunosuppressive tumor microenvironment and immune therapy efficacy indicators.
SPAG5 expression exhibited a negative correlation with most immune cell infiltrates but demonstrated a significant positive correlation with Th2 cells and MDSC cells. Multicolor fluorescence immunohistochemistry demonstrated that SPAG5 activates immune cell populations within tumors, indicating its significant role in the tumor microenvironment. Enrichment analysis indicated that SPAG5-related genes are mainly involved in cell cycle, cellular senescence, P53 signaling pathway, and FoxO signaling pathway. Furthermore, we confirmed the high expression of SPAG5 in cancer cells and observed that its knockdown upregulated the expression of the p53 protein. In conclusion, SPAG5 holds value as a diagnostic, prognostic, and immune biomarker in various cancers and may provide a novel target for tumor immunotherapy.