橡胶树,巴西橡胶树(威尔德。前Adr.deJuss.)穆勒。Arg.,是世界上唯一用于商业生产天然橡胶的工厂。经过多年的繁殖,橡胶树存在广泛的种质分化。橡胶树种质资源多样性和种群结构的探索,在建立核心种质资源的同时,有助于阐明遗传背景并促进这些资源的有效利用和管理。通过使用SNP分子标记技术,扩增了195棵橡胶树资源,他们的遗传多样性分析,随后构建了指纹图谱。通过这个过程,鉴定了橡胶树的耐寒核心种质。结果显示,PIC,他,和pi值分别为0.0905至0.3750、0.095至0.5000和0.0953至0.5013。群体结构分析和聚类分析都将种质划分为两组,表示一个简单的组结构。建立核心种质资源库,抽样比例为10%,由21个种质组成,分为两个种群。G1人口由20个国家组成,而人口G2包括1个登录名。研究结果导致了分子数据库的创建,该数据库有望为橡胶树种质的管理和后续育种应用做出贡献。
The rubber tree, Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg., is the sole plant worldwide utilized for the commercial production of natural rubber. Following years of breeding, there exists a wide array of germplasm differentiation in rubber trees. The exploration of diversity and population structure within rubber tree germplasm resources, alongside the establishment of core germplasm resources, is instrumental in elucidating the genetic background and facilitating the effective utilization and management of these resources. By employing SNP molecular marker technology, 195 rubber tree resources were amplified, their genetic diversity analyzed, and a fingerprint map was subsequently constructed. Through this process, the cold-resistant core germplasm of rubber trees was identified. The results revealed that the PIC, He, and pi values ranged from 0.0905 to 0.3750, 0.095 to 0.5000, and 0.0953 to 0.5013, respectively. Both group structure analysis and cluster analysis delineated the accessions into two groups, signifying a simple group structure. A core germplasm bank was established with a sampling ratio of 10%, comprising 21 accessions divided into two populations. Population G1 consists of 20 accessions, while population G2 comprises 1 accession. The research findings have led to the creation of a molecular database that is anticipated to contribute to the management and subsequent breeding applications of rubber tree accessions.