心力衰竭是由各种因素引起的,使潜在的致病机制难以识别。由于心血管疾病会随着时间的推移而恶化,早期诊断是治疗的关键。此外,了解与衰老相关的心脏质变,关于衰老对心血管疾病的直接影响的信息有限,也将有助于治疗和诊断。为了填补这些研究空白,我们研究的重点是检测与心脏相关的结构和功能分子随时间的变化,专注于聚糖,反映细胞的类型和状态。
方法:我们研究了正常小鼠心脏组织中的聚糖定位及其在衰老过程中的变化,使用渐逝场荧光辅助凝集素微阵列,一种基于凝集素-聚糖相互作用的技术,和凝集素染色。
结果:左心室的聚糖谱显示了腔侧(内侧)和壁侧(外侧)区域之间的差异。中间区域的特征在于存在唾液酸残基。此外,在较年轻的内侧区域观察到与年龄相关的聚糖谱变化.在左心室不同区域,与年龄相关的α-半乳糖水平降低的差异表明微血管数量的时空变化。
结论:聚糖谱,保留了不同的聚糖结构,由许多细胞群体支持,维持心脏功能.随着进一步的研究,聚糖定位和变化有可能被开发为心力衰竭体征的标志物。
Heart failure is caused by various factors, making the underlying pathogenic mechanisms difficult to identify. Since cardiovascular disease tends to worsen over time, early diagnosis is key for treatment. In addition, understanding the qualitative changes in the heart associated with aging, where information on the direct influences of aging on cardiovascular disease is limited, would also be useful for treatment and diagnosis. To fill these research gaps, the focus of our study was to detect the structural and functional molecular changes associated with the heart over time, with a focus on glycans, which reflect the type and state of cells.
METHODS: We investigated glycan localization in the cardiac tissue of normal mice and their alterations during aging, using evanescent-field fluorescence-assisted lectin microarray, a technique based on lectin-glycan interaction, and lectin staining.
RESULTS: The glycan profiles in the left ventricle showed differences between the luminal side (medial) and wall side (lateral) regions. The medial region was characterized by the presence of sialic acid residues. Moreover, age-related changes in glycan profiles were observed at a younger age in the medial region. The difference in the age-related decrease in the level of α-galactose stained with Griffonia simplicifolia lectin-IB4 in different regions of the left ventricle suggests spatiotemporal changes in the number of microvessels.
CONCLUSIONS: The glycan profile, which retains diverse glycan structures, is supported by many cell populations, and maintains cardiac function. With further research, glycan localization and changes have the potential to be developed as a marker of the signs of heart failure.