■DNA甲基化的异常改变被认为是肿瘤发生的标志之一,在急性髓性白血病(AML)的进展中起着至关重要的作用。SMG1是磷酸肌醇3激酶家族的成员,作为抑癌基因。本研究的目的是评估AML中SMG1的表达水平和甲基化状态。
■在这项关于Shariati医院收治的AML患者的后续研究中,德黑兰,伊朗,在三个阶段评估SMG1的甲基化状态[通过甲基化特异性聚合酶链反应(PCR)进行]及其表达水平(通过qRT-PCR进行):新诊断,正在治疗和完全缓解。SMG1的甲基化状态、其表达水平、并通过SPSSver.25对临床/临床数据进行分析。
■这项对18例患者和5例对照个体的研究表明,与正常组相比,新诊断病例中SMG1启动子的CpG岛被低甲基化(P=0.002)。新病例中SMG1表达水平的倍数变化为0.464±0.468,而治疗不足和缓解期患者中SMG1表达水平的倍数变化分别为0.9159±0.873和0.685±0.885,在治疗不足的患者中,白细胞(WBC)计数减少114176.36细胞/μl,每增加一个单位的SMG1倍数变化(P<0.0001),Hb单位随每单位倍数的增加而增加2.062g/dl(P<0.0001)。此外,在缓解阶段,Hb单元随每个单元的倍数变化而增加1.395g/dl(P=0.019)。
■我们研究的有力结果表明,的甲基化和表达对AML的发病机理有很大影响。此外,SMG1的甲基化和表达可能在AML中发挥预后作用。
UNASSIGNED: Aberrant alterations in DNA methylation are known as one of the hallmarks of oncogenesis and play a vital role in the progression of acute myeloid leukemia (AML).
SMG1 is a member of the Phosphoinositide 3-kinases family, acting as a tumor suppressor gene. The aim of this study was the evaluation of the expression level and methylation status of
SMG1 in AML.
UNASSIGNED: In this follow-up study on AML patients admitted to Shariati Hospital, Tehran, Iran, the methylation status of SMG1 [performed by methylation-specific polymerase chain reaction (PCR)] and its expression level (performed by qRT-PCR) were evaluated in three phases: newly diagnosed, under treatment and complete remission. The correlation of the methylation status of
SMG1, its expression level, and clinical/paraclinical data was analyzed by SPSS ver.25.
UNASSIGNED: This study on 18 patients and five control individuals showed that the CpG-islands of the SMG1 promoter in newly diagnosed cases is hypomethylated compared to the normal group (P=0.002) The fold change of SMG1 expression levels in new cases is 0.464 ± 0.468, while the fold change of
SMG1 expression levels in under-treatment and in-remission patients is 0.973 ± 1.159 and 0.685 ± 0.885, respectively. In under-treatment patients, white blood cell (WBC) count decreases 114176.36 cell/μl with each unit of increase in fold change of SMG1 (P<0.0001), and Hb unit increases 2.062 g/dl with each unit of increase in fold change (P<0.0001). Also, in the remission phase, the Hb unit increases 1.395 g/dl with each unit increase in fold change (P=0.019).
UNASSIGNED: The robust results of our study suggest that the methylation and expression of have a high impact on the pathogenesis of AML. Also, the methylation and expression of
SMG1 can play a prognostic role in AML.