UNASSIGNED:通过免疫组织化学评估PAX8在正常儿童和成人晶状体中的表达,并评估PAX8免疫组织化学染色在诊断晶状体起源的形态学上具有挑战性的病变中的有用性。
未经批准:回顾性,观察案例系列。
未经证实:14个先天性和获得性晶状体源性病变和10个对照晶状体。
未经证实:苏木精-伊红和高碘酸-希夫染色和PAX8波形蛋白的免疫组织化学面板,S100,平滑肌肌动蛋白,在所有组织上进行AE1/AE3、细胞角蛋白7和细胞角蛋白5/6抗体。
未经证实:PAX8在正常晶状体和晶状体源性病变中的表达分布.
未经评估:记录搜索确定了10个正常的儿童和成人晶状体,1个phokomatous脉络膜瘤,1Peters异常伴粘附性白血病,1晶状体囊与先天性锥体性白内障形成,2晶状体前、后囊下白内障形成,3术后白内障晶状体(Soemmerring环白内障和囊膜纤维化),和6个结合了化生角膜内皮各种成分的角膜后膜,晶状体上皮,角膜基质,和上皮向下生长。在正常小儿和成人晶状体的晶状体上皮和赤道晶状体弓中观察到强烈的核PAX8表达。在保留一些上皮形态特征的病变中也观察到核PAX8表达,如phokomatous脉络膜瘤,粘附性白细胞瘤,先天性锥体白内障,和晶状体上皮分化的眼膜成分。PAX8表达在经历间质转化的晶状体上皮病变中丢失,如前囊膜下白内障和囊膜纤维化。
UASSIGNED:PAX8抗体可能是形态学上具有挑战性的晶状体上皮源性病变的免疫组织化学面板的有用辅助手段。PAX8在以上皮-间质转化为特征的晶状体源性病变的诊断中没有用。
UNASSIGNED: To evaluate PAX8 expression by immunohistochemistry in the normal pediatric and adult crystalline lens and to assess the usefulness of PAX8 immunohistochemical stain in the diagnosis of morphologically challenging lesions of lenticular origin.
UNASSIGNED: Retrospective, observational case series.
UNASSIGNED: Fourteen congenital and acquired lens-derived lesions and 10 control crystalline lenses.
UNASSIGNED: Hematoxylin-eosin and periodic acid-Schiff stains and an immunohistochemical panel of PAX8, vimentin, S100, smooth muscle actin, AE1/AE3, cytokeratin 7, and cytokeratin 5/6 antibodies were performed on all tissues.
UNASSIGNED: Distribution of PAX8 expression in normal crystalline lens and in lens-derived lesions.
UNASSIGNED: Records search identified 10 normal pediatric and adult crystalline lenses, 1 phakomatous choristoma, 1 Peters anomaly with adherent leukoma, 1 lens capsule with congenital pyramidal cataract formation, 2 lenses with anterior and posterior subcapsular cataract formation, 3 postsurgical cataractous lenses (Soemmerring ring cataract and capsular fibrosis), and 6 retrocorneal membranes that incorporated various components of metaplastic corneal endothelium, metaplastic lens epithelium, corneal stroma, and epithelial downgrowth. Strong nuclear PAX8 expression was observed in the lens epithelium and in the equatorial lens bow of normal pediatric and adult lenses. Nuclear PAX8 expression also was observed in the lesions that retained some of the epithelial morphologic features, such as phakomatous choristoma, adherent leukoma, congenital pyramidal cataract, and components of intraocular membranes with lens epithelial differentiation. PAX8 expression was lost in lens epithelial lesions that had undergone mesenchymal transition, such as anterior subcapsular cataract and capsular fibrosis.
UNASSIGNED: PAX8 antibody may be a useful adjunct to the immunohistochemical panels in morphologically challenging lens epithelial-derived lesions that retain epithelial morphologic features. PAX8 is not useful in the diagnosis of lens-derived lesions that feature epithelial-mesenchymal transition.