纽波特沙门氏菌(S.Newport)是与人沙门氏菌病相关的主要血清型。在中国,从人类和其他来源回收的总共79个纽波特被鉴定为抗菌药物敏感性。使用脉冲场凝胶电泳(PFGE)的毒力基因谱和分子亚型。大约63.3%的分离株对所测试的所有16种抗微生物剂敏感。近三分之一的分离株(31.6%)对硫异恶唑耐药,20.3%为四环素,13.9%为萘啶酸。12个分离株(15.2%)对三种或更多种抗菌剂具有抗性。在检测到的10个毒力基因中,沙门氏菌致病岛基因avrA,ssaQ,mgtC,siiD,sopB和菌毛基因bcfC在大多数分离株中存在(93.7%至100%)。总的来说,我们观察到9个不同的毒力基因谱,其中3种(VP1、VP2和VP3)最常见(86.1%)。总共确定了56个PFGE模式,主要分为7个簇(A到G),模式相似性为80%。来自水产品的分离物与来自人类的分离物具有很高的相似性,强调水产品作为S.Newport感染人类的来源的潜在风险。此外,某些PFGE簇和毒力基因图谱之间有很强的关联,提示毒力分型可作为鉴别S.Newport分离株的有用流行病学工具。
Salmonella Newport (S. Newport) is a major serotype associated with human salmonellosis. A total of 79 S. Newport recovered from humans and other sources in China were characterized for antimicrobial susceptibility, virulence gene profiles and molecular subtypes using pulsed field gel electrophoresis (PFGE). Approximately 63.3% of the isolates were susceptible to all of 16 antimicrobials tested. Nearly one third of the isolates (31.6%) were resistant to sulfisoxazole, 20.3% to tetracycline and 13.9% to nalidixic acid. Twelve isolates (15.2%) were resistant to three or more antimicrobials. Among 10 virulence genes detected, Salmonella pathogenicity island genes avrA, ssaQ, mgtC, siiD, and sopB and fimbrial gene bcfC were present in most of the isolates (93.7% to 100%). Overall, we observed nine distinct virulence gene profiles, three of which (VP1, VP2 and VP3) were most common (86.1%). A total of 56 PFGE patterns were identified and mainly grouped into seven clusters (A to G) with 80% pattern similarity. Isolates from aquatic product shared a high similarity with those from humans in several clusters, highlighting a potential risk of aquatic product as a source of S. Newport that infect humans. Furthermore, there was a strong association between certain PFGE clusters and virulence gene profiles, suggesting virulence subtyping can be a useful epidemiological tool to discriminate S. Newport isolates.