禽类沙门氏菌病伴随着家禽业的高度金融危机以及人类的食源性疾病。本研究旨在研究肠炎沙门氏菌和鼠伤寒沙门氏菌在患病肉鸡中的出现,电阻剖面,监测毒力和抗生素抗性基因。因此,450个样本(泄殖腔拭子,肝脏,和脾脏)是从吉萨省不同农场的150只患病鸟类中收集的,埃及。随后,做了细菌学检查.之后,对获得的沙门氏菌分离株进行了血清分组测试,抗菌谱,PCR监测毒力(invA,stn,hila,和PEFA),和抗菌素抗性基因(blaTEM,BlaCTX-M,blaNDM,ermA,sul1,tetA,和aadA1)。沙门氏菌在被检查的患病肉鸡中的总患病率为9.3%,在泄殖腔拭子中患病率最高。在回收的沙门氏菌分离株(n=35)中,20个血清型被识别为肠炎沙门氏菌,15个血清型被识别为鼠伤寒沙门氏菌。几乎60%的检索到的S.肠炎血清变型对七个抗菌类别广泛耐药(XDR),并遗传sul1,blaTEM,tetA,BlaCTX-M,erea,和aadA1基因。同样,25%的回收的肠炎链球菌血清变型对6类具有多重耐药(MDR),并具有sul1、blaTEM、tetA,BlaCTX-M,和erea抗性基因。此外,66.7%的检索到的S.鼠伤寒菌血清型XDR为7类,并具有sul1,blaTEM,tetA,BlaCTX-M,erea,和aadA1基因。简洁地,这份报告强调了XDR鼠伤寒沙门氏菌和S.肉鸡的肠炎。美罗培南和诺氟沙星对重新出现的XDR鼠伤寒沙门氏菌和S.肉鸡肠炎。此外,这些XDR沙门氏菌菌株的复发构成了潜在的公共卫生威胁.
Avian salmonellosis is concomitant with high financial crises in the poultry industry as well as food-borne illness in man. The present study is designed to investigate the emergence of Salmonella Enteritidis and Salmonella Typhimurium in diseased broilers, resistance profiles, and monitoring virulence and antibiotic resistance genes. Consequently, 450 samples (cloacal swabs, liver, and spleen) were collected from 150 diseased birds from different farms in Giza Governorate, Egypt. Subsequently, the bacteriological examination was done. Afterward, the obtained Salmonella isolates were tested for serogrouping, antibiogram, PCR monitoring of virulence (invA, stn, hilA, and pefA), and antimicrobial resistance genes (blaTEM, blaCTX-M, blaNDM, ermA, sul1, tetA, and aadA1). The total prevalence of Salmonella in the examined diseased broilers was 9.3%, and the highest prevalence was noticed in cloacal swabs. Among the recovered Salmonella isolates (n = 35), 20 serovars were recognized as S. Enteritidis and 15 serovars were identified as S. Typhimurium. Almost 60% of the retrieved S. Enteritidis serovars were extensively drug-resistant (XDR) to seven antimicrobial classes and inherited sul1, blaTEM, tetA, blaCTX-M, ereA, and aadA1 genes. Likewise, 25% of the recovered S. Enteritidis serovars were multidrug-resistant (MDR) to six classes and have sul1, blaTEM, tetA, blaCTX-M, and ereA resistance genes. Also, 66.7% of the retrieved S. Typhimurium serovars were XDR to seven classes and have sul1, blaTEM, tetA, blaCTX-M, ereA, and aadA1 genes. Succinctly, this report underlined the reemergence of XDR S. Typhimurium and S. Enteritidis in broiler chickens. Meropenem and norfloxacin exposed a hopeful antimicrobial activity toward the re-emerging XDR S. Typhimurium and S. Enteritidis in broilers. Moreover, the recurrence of these XDR Salmonella strains poses a potential public health threat.