低温(冷)胁迫是影响全球作物生产力的主要非生物胁迫条件之一。一氧化氮(NO)是一种动态信号分子,与各种胁迫调节剂相互作用并提供非生物胁迫耐受性。应激增强的NO有助于S-亚硝基硫醇的积累,从而导致蛋白质中-SH基团的氧化,从而导致S-亚硝化。翻译后修饰。冷应激诱导的体内>240种蛋白质的S-亚硝化主要属于应激/信号/氧化还原(黑芥子酶,SOD,GST,CS,DHAR),光合作用(RuBisCO,PRK),新陈代谢(FBA,GAPDH,TPI,SBPase),和细胞壁修饰(β-木糖苷酶,α-1-阿拉伯半乳聚糖)在不同作物中表明NO在这些重要的细胞和代谢途径中的作用。NO介导的转录因子CBF(C-重复结合因子,转录因子)在番茄幼苗中显示了转录和翻译后水平。NO供体引发增强种子萌发,打破休眠并提供对作物胁迫的耐受性。它在避免压力方面的作用,促进种子发芽,延缓衰老为使用NO和释放NO的化合物以防止作物损失和增加水果和蔬菜的保质期铺平了道路。替代能源消耗和昂贵的冷藏导致开发了一种称为“保质期增强剂”的存储设备,该设备可以使用NO供体在环境温度(25-27°C)下延迟衰老并增加保质期。本综述总结了植物中NO的研究,并探索了NO在提高农业产量和收获后作物损失方面的转化潜力。
■在线版本包含补充材料,可在10.1007/s12298-023-01371-z获得。
Low temperature (cold) stress is one of the major abiotic stress conditions affecting crop productivity worldwide. Nitric oxide (NO) is a dynamic signaling molecule that interacts with various stress regulators and provides abiotic stress tolerance. Stress enhanced NO contributes to S-nitrosothiol accumulation which causes oxidation of the -SH group in proteins leading to S-nitrosation, a post-translational modification. Cold stress induced in vivo S-nitrosation of > 240 proteins majorly belonging to stress/signaling/redox (myrosinase, SOD, GST, CS, DHAR), photosynthesis (RuBisCO, PRK), metabolism (FBA, GAPDH, TPI, SBPase), and cell wall modification (Beta-xylosidases, alpha-l-arabinogalactan) in different crop plants indicated role of NO in these important cellular and metabolic pathways. NO mediated regulation of a transcription factor CBF (C-repeat Binding Factor, a transcription factor) at transcriptional and post-translational level was shown in Solanum lycopersicum seedlings. NO donor priming enhances seed germination, breaks dormancy and provides tolerance to stress in crops. Its role in averting stress, promoting seed germination, and delaying senescence paved the way for use of NO and NO releasing compounds to prevent crop loss and increase the shelf-life of fruits and vegetables. An alternative to energy consuming and expensive cold storage led to development of a storage device called \"shelf-life enhancer\" that delays senescence and increases shelf-life at ambient temperature (25-27 °C) using NO donor. The present review summarizes NO research in plants and exploration of NO for its translational potential to improve agricultural yield and post-harvest crop loss.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-023-01371-z.