蛋白S-酰基转移酶(PATs)催化的蛋白S-酰化是一种可逆的脂质修饰,稳定性,和互动档案。PAT由植物中的大型基因家族编码,和许多蛋白质,包括受体样细胞质激酶(RLCKs)和受体样激酶(RLKs)经历S-酰化。然而,很少有PAT被分配了底物,和少数S-酰化蛋白具有已知的上游酶。我们报道拟南芥(拟南芥)A类PAT冗余地介导花粉管引导,并参与花粉受体激酶1(PRK1)的S-酰化和在花粉管引导1(LIP1)中的丢失,用于花粉管引导的临界RLK或RLCK,分别。PAT1、PAT2、PAT3、PAT4和PAT8,为简单起见统称为PENTAPAT,富含花粉,并显示相似的亚细胞分布。PENTAPAT的功能丧失减少了由于雄配子体缺陷引起的结实率。具体来说,五子叶花粉管在定向生长中受到损害。我们确定PRK1和LIP1与PENTAPAT相互作用,并且它们的S-酰化在五氮花粉中减少。五子叶花粉中LIP1的质膜(PM)缔合减少,而减少PRK1S-酰化的点突变影响其与其相互作用蛋白的亲和力。我们的结果表明S-酰化通过调节PM受体复合物在花粉管引导中的关键作用。
Protein S-acylation catalyzed by protein S-acyl transferases (PATs) is a reversible lipid modification regulating protein targeting, stability, and interaction profiles. PATs are encoded by large gene families in plants, and many proteins including receptor-like cytoplasmic kinases (RLCKs) and receptor-like kinases (RLKs) are subject to S-acylation. However, few PATs have been assigned substrates, and few S-acylated proteins have known upstream enzymes. We report that Arabidopsis (Arabidopsis thaliana) class A PATs redundantly mediate pollen tube guidance and participate in the S-acylation of POLLEN RECEPTOR KINASE1 (PRK1) and LOST IN POLLEN TUBE GUIDANCE1 (LIP1), a critical RLK or RLCK for pollen tube guidance, respectively. PAT1, PAT2, PAT3, PAT4, and PAT8, collectively named PENTAPAT for simplicity, are enriched in pollen and show similar subcellular distribution. Functional loss of PENTAPAT reduces seed set due to male gametophytic defects. Specifically, pentapat pollen tubes are compromised in directional growth. We determine that PRK1 and LIP1 interact with PENTAPAT, and their S-acylation is reduced in pentapat pollen. The plasma membrane (PM) association of LIP1 is reduced in pentapat pollen, whereas point mutations reducing PRK1 S-acylation affect its affinity with its interacting proteins. Our results suggest a key role of S-acylation in pollen tube guidance through modulating PM receptor complexes.