背景:肠道菌群可通过调节代谢和免疫途径影响血管性认知障碍(VCI)的发生和发展。然而,所涉及的血管机制尚不清楚.
目的:研究与VCI相关的肠道菌群,并研究局部脑血流量(CBF)的中介作用,以探索VCI的潜在治疗靶点。
方法:这项前瞻性研究纳入了VCI患者(n=16)和健康对照(n=18),生物标志物,以及2022年1月1日至6月30日之间的生活方式研究。通过16S核糖体RNA基因测序确定肠道菌群组成和多样性。使用Spearman相关分析确定肠道微生物群与蒙特利尔认知评估(MoCA)评分之间的关联。使用伪连续动脉自旋标记计算区域CBF。利用中介分析研究了区域CBF在VCI中特定肠道菌群与认知之间关系的中介作用。
结果:与健康对照组相比,VCI患者的双歧杆菌丰度明显更高,Veillonella,雀巢R,梭杆菌,和Eryselatoclostridium和较少的Collinsella丰度。与左下丘脑的CBF呈负相关的是,在VCI患者的MoCA评分中,右下丘脑,左杏仁核占63.96%,48.22%,和36.51%,分别,在对混杂因素进行调整后,这种关联的。
结论:经双侧下丘脑和左杏仁核的CBF强烈介导,与VCI的认知有关。这些发现强调了营养和代谢相关区域在VCI中的潜在调节作用。
Gut microbiota could affect the onset and development of vascular cognitive impairment (VCI) through modulating metabolic and immune pathways. However, the vascular mechanisms involved remain unclear.
To investigate the gut microbiota associated with VCI and examine the mediating effects of regional cerebral blood flow (CBF) to explore potential therapeutic targets for VCI.
This prospective study enrolled patients with VCI (n = 16) and healthy controls (n = 18) from the Chinese Imaging, Biomarkers, and Lifestyle study between January 1 and June 30, 2022. The gut microbiota composition and diversity were determined by 16 S ribosomal RNA gene sequencing. The association between gut microbiota and Montreal Cognitive Assessment (MoCA) scores was determined using Spearman\'s correlation analysis. Regional CBF was calculated using pseudo-continuous arterial spin labeling. The mediating effects of regional CBF on the relationship between specific gut microbiota and cognition in VCI were investigated using mediation analysis.
Compared to healthy controls, patients with VCI had significantly greater abundance of Bifidobacterium, Veillonella, R uminococcus gnavus , Fusobacterium, and Erysipelatoclostridium and smaller abundance of Collinsella. The abundance of Ruminococcus gnavus was negatively associated with MoCA scores in patients with VCI, with the CBF in the left hypothalamus, right hypothalamus, and left amygdala accounting for 63.96%, 48.22%, and 36.51%, respectively, of this association after adjusting for confounders.
Ruminococcus gnavus is associated with cognition in VCI, which is strongly mediated by CBF in the bilateral hypothalamus and left amygdala. These findings highlight the potential regulatory roles of nutrition and metabolism-related areas of the brain in VCI.