为了实现厌氧氨氧化污泥长期常温贮存后细菌活性的快速恢复,将三组反应器添加到室温(15-30℃)无底物储存9个月的厌氧氨氧化污泥中。在三组反应堆中,将彗星纤维载体和K3载体添加到R2和R3反应器中,分别,作为生物载体。研究了不同载体对厌氧氨氧化污泥细菌活性回收率的影响。结果表明,R2和R3反应器中的厌氧氨氧化反应在第8天和第10天开始发生,分别,TIN去除率分别为82.25%和80.92%,与R1反应器相比,其中未添加载体(ANAMMOX反应在第15天开始发生,TIN去除率为80.26%)。有进水42天后,ρ(NH4+-N)和ρ(NO2--N)分别提高到300mg·L-1和396mg·L-1,三组反应器的TIN去除率分别为78.96%,84.92%,和84.66%。微生物群落结构分析表明,在R2和R3反应器中厌氧氨氧化菌的相对丰度分别为6.85%和6.06%,是R1反应器的两到四倍。污泥中主要的厌氧氨氧化细菌是Jettenia念珠菌,其在三组反应堆中的相对丰度分别为1.62%,5.74%,5.21%。结果表明,通过添加载体构建的ANAMMOX生物膜-颗粒污泥复合系统可以大大缩短无底物长期室温储存后恢复ANAMMOX污泥细菌活性的时间。载体有效地促进了反应器中厌氧氨氧化菌的相对丰度,而彗星纤维载体的促进作用比K3载体稍显著。
In order to realize the rapid recovery of ANAMMOX sludge bacterial activity after long-term room temperature storage, three groups of reactors were added to ANAMMOX sludge that had been stored without substrate at room temperature (15-30℃) for 9 months. Among the three groups of reactors, comet fiber carrier and K3 carrier were added to R2 and R3 reactors, respectively, as biological carriers. The effects of different carriers on the recovery rate of ANAMMOX sludge bacterial activity were investigated. The results showed that ANAMMOX reactions in the R2 and R3 reactors began taking place on the 8th and 10th day, respectively, with respective TIN removal rates of 82.25% and 80.92%, which were significantly improved compared with that in the R1 reactor, in which no carrier was added (ANAMMOX reaction started occurring on the 15th day with a TIN removal rate of 80.26%). After 42 days with influent, ρ(NH4+-N) and ρ(NO2--N) respectively increased to 300 mg·L-1 and 396 mg·L-1, and the TIN removal rates of the three groups of reactors were respectively 78.96%, 84.92%, and 84.66%. Microbial community structure analysis showed that the relative abundances of ANAMMOX bacteria in the R2 and R3 reactor were respectively 6.85% and 6.06%, two to four times that in the R1 reactor. The predominant ANAMMOX bacteria in the sludge was Candidatus Jettenia, whose relative abundances in the three groups of reactors were respectively 1.62%, 5.74%, and 5.21%. The results show that ANAMMOX biofilm-granular sludge complex systems constructed by adding carriers can considerably shorten the time for recovering ANAMMOX sludge bacterial activity after long-term room temperature storage without substrate. The carriers effectively promoted the relative abundances of ANAMMOX bacteria in the reactors, whereas the promoting effect of comet fiber carrier was slightly more significant than that of the K3 carrier.