背景:我们先前的研究揭示了核糖体蛋白(RP)基因在水稻非生物胁迫反应中的作用。
方法:在当前的调查中,我们研究了这些基因可能参与昆虫应激反应。我们已经表征了RP基因,该基因包括核糖体蛋白大(RPL)和核糖体蛋白小(RPS)亚基基因,以响应两种经济上重要的昆虫害虫的侵染。稻中的褐飞虱(BPH)和亚洲稻胆mid(GM)。研究了70个选定的RP基因的差异转录模式,分别是in稻的易感和抗性基因型:BPT5204和RPNF05。这些基因的上游区域的计算机模拟分析也揭示了与伤口信号传导相关的顺式元件的存在。
结果:我们确定了在任一基因型中上调或下调的基因,或者在害虫侵扰后两者兼而有之。发现大多数基因的转录模式受两种害虫的时间调节。在抗性RPNF05中,BPH侵染激活了RPL15,L51和RPS5a基因,而GM侵染诱导了RPL15,L18a,L22、L36.2、L38、RPS5、S9.2和S25a在某个时间点。这些基因在抗性基因型中特别上调,RPNF05,但在BPT5204中没有表明它们可能参与植物对所研究的两种害虫中的任何一种的抗性。
结论:综合来看,RPL15,L51,L18a,RPS5,S5a,S9.2和S25a似乎是可能在水稻抗虫性中起作用的基因。
BACKGROUND: Our previous studies have revealed the roles of ribosomal protein (RP) genes in the abiotic stress responses of rice.
METHODS: In the current investigation, we examine the possible involvement of these genes in insect stress responses. We have characterized the RP genes that included both Ribosomal Protein Large (RPL) and Ribosomal Protein Small (RPS) subunit genes in response to infestation by two economically important insect pests, the brown planthopper (BPH) and the Asian rice gall midge (GM) in rice. Differential transcript patterns of seventy selected RP genes were studied in a susceptible and a resistant genotype of indica rice: BPT5204 and RPNF05, respectively. An in silico analyses of the upstream regions of these genes also revealed the presence of cis-elements that are associated with wound signaling.
RESULTS: We identified the genes that were up or downregulated in either one of the genotypes, or both of them after pest infestation. The transcript patterns of a majority of the genes were found to be temporally-regulated by both the pests. In the resistant RPNF05, BPH infestation activated RPL15, L51 and RPS5a genes while GM infestation induced RPL15, L18a, L22, L36.2, L38, RPS5, S9.2 and S25a at a certain point of time. These genes that were particularly upregulated in the resistant genotype, RPNF05, but not in BPT5204 suggest their potential involvement in plant resistance against either of the two pests studied.
CONCLUSIONS: Taken together, RPL15, L51, L18a, RPS5, S5a, S9.2, and S25a appear to be the genes with possible roles in insect resistance in rice.