人为污染,包括最初旨在解决粮食安全和医疗保健问题的绿色革命产生的残留物,矛盾的是加剧了环境挑战。向全面的绿色生物技术和生物修复过渡,通过较低的金融投资来实现,取决于微生物生物技术,红球菌属成为有希望的竞争者。完全注释基因组序列的意义在于理解菌株成分,设计实验方案,并战略性地部署这些菌株以使用关键基因解决相关问题。这项研究围绕红球红球菌MGMM8展开,这是根际冬小麦植物的关联。经由过程对其染色体基因组的注释和后续与其他菌株的比拟,探讨了其潜在的应用。使用antiSMASH服务器,预测了19个基因簇,包括负责抗生素和铁载体的基因。通过综合抗生素耐药性数据库(CARD)进行的抗生素耐药性评估确定了五个基因(vanW,vany,RbpA,iri,和folC)与菌株CCM2595平行。利用NCBI原核基因组注释管道(PGAP)进行生物降解,耐重金属,和修复基因,氯胺隆乙基的存在,甲醛,苯脱硫降解基因,和重金属相关基因(ACR3,ARsC,科拉,Dsba,moda,并确认了MGMM8中的recG)。此外,群体猝灭信号基因,对于抑制病原体群体感应引起的生物膜形成和毒力至关重要,在MGMM8的基因组中也被识别。根据这些预测,新型分离株MGMM8需要进行表型评估,以评估其在生物防治和生物修复中的潜力。该评价延伸到分离针对病原微生物的潜在抗微生物活性的活性化合物。全面的基因组注释过程促进了MGMM8的遗传表征,并巩固了其作为解决全球人为困境的生物技术菌株的潜力。
Anthropogenic pollution, including residues from the green revolution initially aimed at addressing food security and healthcare, has paradoxically exacerbated environmental challenges. The transition towards comprehensive green biotechnology and bioremediation, achieved with lower financial investment, hinges on microbial biotechnology, with the Rhodococcus genus emerging as a promising contender. The significance of fully annotating genome sequences lies in comprehending strain constituents, devising experimental protocols, and strategically deploying these strains to address pertinent issues using pivotal genes. This study revolves around Rhodococcus erythropolis MGMM8, an associate of winter wheat plants in the rhizosphere. Through the annotation of its chromosomal genome and subsequent comparison with other strains, its potential applications were explored. Using the antiSMASH server, 19 gene clusters were predicted, encompassing genes responsible for antibiotics and siderophores. Antibiotic resistance evaluation via the Comprehensive Antibiotic Resistance Database (CARD) identified five genes (vanW, vanY, RbpA, iri, and folC) that were parallel to strain CCM2595. Leveraging the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) for biodegradation, heavy metal resistance, and remediation genes, the presence of chlorimuron-ethyl, formaldehyde, benzene-desulfurization degradation genes, and heavy metal-related genes (ACR3, arsC, corA, DsbA, modA, and recG) in MGMM8 was confirmed. Furthermore, quorum-quenching signal genes, critical for curbing biofilm formation and virulence elicited by quorum-sensing in pathogens, were also discerned within MGMM8\'s genome. In light of these predictions, the novel isolate MGMM8 warrants phenotypic assessment to gauge its potential in biocontrol and bioremediation. This evaluation extends to isolating active compounds for potential antimicrobial activities against pathogenic microorganisms. The comprehensive genome annotation process has facilitated the genetic characterization of MGMM8 and has solidified its potential as a biotechnological strain to address global anthropogenic predicaments.