两个新颖的菌株,指定APW6T和APW11T,与人工池塘水隔离,和一种新颖的菌株,指定PFR6T,是从中提琴根中分离的。这些菌株被发现是革兰氏阴性的,杆状,通过鞭毛运动,和氧化酶阳性.类型菌株的生长条件如下:APW6T,15-43°C(最佳,28°C),pH6.0-12.0(最佳,pH7.0),没有盐度;APW11T,4-35°C(最佳,25°C),pH6.0-11.0(最佳,pH9.0),含0-1%NaCl(w/v,最佳0%);PFR6T,10-38°C(最佳28°C),pH6.0-12.0(最佳,pH7.0),用0-2%NaCl(w/v;最佳,0%)。菌株APW6T,APW11T,PFR6T属于玫瑰属,16SrRNA基因序列相似性最强,与萨氏玫瑰病DSM654T(98.1%),玫瑰寡养型CHU3T(98.7%),和玫瑰puraquaeCCUG52769T(98.1%)。估计APW6T的基因组大小,APW11T,PFR6T分别为5050473、5670008和5216869bp,G+C含量分别为69.5、66和68.5mol%。数字DNA-DNA杂交,平均氨基酸同一性,新菌株和相关分类群的平均核苷酸同一性值均低于22.4、74.7和78.9%,分别。所有菌株的主要细胞脂肪酸(>10%)为特征3(包括C16:1ω6c和/或C16:1ω7c)和C16:0。PFR6T还具有总计特征8(包括C18:1ω7c和/或C18:1ω6c)作为主要脂肪酸。所有菌株的极性脂质谱都含有磷脂酰乙醇胺,磷酸氨基糖脂,和磷酸糖脂.独特的系统发育,生理,本研究报告的化学分类学特征表明,菌株APW6T,APW11T,和PFR6T代表玫瑰属中的新物种,为其命名玫瑰亚黄sp。11月。,应变类型为APW6T(=KACC22877T=TBRC16606T),水红玫瑰。11月。,应变类型为APW11T(=KACC22878T=TBRC16607T),和紫罗兰玫瑰。分别提出了nov(=KACC23257T=TBRC17653T)。
Two novel strains, designated APW6T and APW11T, were isolated from artificial pond water, and one novel strain, designated PFR6T, was isolated from a Viola mandshurica root. These strains were found to be Gram-negative, rod-shaped, motile by means of flagella, and oxidase-positive. Growth conditions of the type strains were as follows: APW6T, 15-43 °C (optimum, 28 °C), pH 6.0-12.0 (optimum, pH 7.0), with no salinity; APW11T, 4-35 °C (optimum, 25 °C), pH 6.0-11.0 (optimum, pH 9.0), with 0-1 % NaCl (w/v, optimum 0 %); PFR6T, 10-38 °C (optimum 28 °C), pH 6.0-12.0 (optimum, pH 7.0), with 0-2 % NaCl (w/v; optimum, 0 %). Strains APW6T, APW11T, and PFR6T belonged to the genus Roseateles, having the most 16S rRNA gene sequence similarity to Roseateles saccharophilus DSM 654T (98.1 %), Roseateles oligotrophus CHU3T (98.7 %), and Roseateles puraquae CCUG 52769T (98.1 %). The estimated genome sizes of APW6T, APW11T, and PFR6T were 50 50 473, 56 70 008, and 52 16 869 bp, respectively and the G+C contents were 69.5, 66, and 68.5 mol%. The digital DNA-DNA hybridization, average amino acid identity, and average nucleotide identity values among the novel strains and related taxa were all lower than 22.4, 74.7, and 78.9 %, respectively. The predominant cellular fatty acids (>10 %) of all strains were summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c) and C16 : 0. PFR6T also had summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as a major fatty acid. The polar lipid profile of all strains contained phosphatidylethanolamine, phosphoaminoglycolipid, and phosphoglycolipid. The distinct phylogenetic, physiological, and chemotaxonomic features reported in this study indicate that strains APW6T, APW11T, and PFR6T represent novel species within the genus Roseateles, for which the names Roseateles subflavus sp. nov., with the type strain APW6T (=KACC 22877T=TBRC 16606T), Roseateles aquae sp. nov., with the type strain APW11T (=KACC 22878T=TBRC 16607T), and Roseateles violae sp. nov (=KACC 23257T=TBRC 17653T) are respectively proposed.