在这项研究中,我们在韩国玫瑰苦味中诱导了四倍体,Rhodeusuyekii,通过施加各种静水压力冲击条件。在任何实验组中在4500psi压力处理下均未诱导四倍体。相反,在7500psi静水压力处理下,四倍体的诱导率最高。因此,当处理方法相似并且随着处理时间的增加,每个实验组的诱导率增加;然而,差异无统计学意义(P>0.05)。生产率为3.1%,在受精100分钟后暴露于6000psi10分钟的所有实验组中最高。在单独使用静水压力处理的实验组中,生产率最高。而在静水压力下用化学处理处理的组中,生产率最低。7500psi处理20min的所有实验组的异常率非常高,在5%左右。基于这些研究,根据计算的孵化,只有静水压力冲击被认为是有效诱导四倍体,异常,和诱导率。诱导四倍体的最有效条件是施肥100分钟后,静水压力冲击10分钟为6000psi。诱导的四倍体韩国玫瑰苦味的染色体数为4n=96,而二倍体的染色体数为2n=48。在二倍体中,细胞里有1或2个核仁,而诱导的四倍体包含1、2、3或4。四倍体和二倍体的DNA含量分别为3.68±0.009pg/核和1.84±0.019pg/核,分别,根据流式细胞仪分析。四倍体的DNA含量和染色体数目是二倍体的两倍。
In this study, we induced tetraploidy in Korean rose bitterling, Rhodeus uyekii, by applying various hydrostatic pressure shock conditions. Tetraploidy was not induced under 4500 psi pressure treatment in any experimental group. Instead, the induction rate of tetraploidy was highest under 7500 psi hydrostatic pressure treatment. As a result, when the processing method was similar and as the process time increased, the induction rate of each experimental group increased; however, there was no significant difference (P > 0.05). The production rate was 3.1 %, which was highest in all experimental groups exposed to 6000 psi for 10 min after being fertilized for 100 min. The production rate was highest in the experimental groups treated with hydrostatic pressure alone, whereas the production rate was lowest in groups treated under hydrostatic pressure with chemical treatment. The abnormal rate of all experimental groups treated with 7500 psi for 20 min was very high, at about 5 %. Based on these studies, only hydrostatic pressure shock was considered effective at inducing tetraploidy based on the calculated hatching, abnormal, and induction rates. The most effective condition for inducing tetraploidy was 6000 psi of hydrostatic pressure shock for 10 min after being fertilized for 100 min. The chromosome number of the induced tetraploid Korean rose bitterling was 4n = 96, while that of the diploid was 2n = 48. In the diploid, there were 1 or 2 nucleoli in the cells, whereas the induced tetraploids contained 1, 2, 3, or 4. The DNA content of tetraploids and diploids were 3.68 ± 0.009 pg/nucleus and 1.84 ± 0.019 pg/nucleus, respectively, according to flow cytometric analysis. The DNA content and chromosome number of the tetraploids were twice that of the diploids.