植物不再被视为独立实体;相反,它们拥有多种促进植物生长的根际细菌(PGPR),可帮助它们获得养分,并且还可以提供恢复力。宿主植物以菌株特异性方式识别PGPR;因此,引入非靶向PGPR可能会产生不令人满意的作物产量。因此,开发一种微生物辅助贯叶连翘栽培技术,从该植物的高海拔印度西部喜马拉雅自然栖息地中分离出31种根际细菌,并在体外表征了多种植物生长促进属性。在31个根瘤菌分离物中,26产生0.59至85.29μgmL-1吲哚-3-乙酸,溶解15.77至71.43μgmL-1无机磷酸盐;21产生63.12至99.92%的铁载体单位,和15表现出103.60至1,296.42nmolα-酮丁酸酯mg-1蛋白h-11-氨基环丙烷-1-羧酸脱氨酶(ACCD)活性。基于优越的植物生长促进属性,在多温室条件下,进一步评估了八个具有统计学意义的多种PGPR,用于植物中植物生长促进试验。用KosakoniacowaniiHypNH10和RahnellavariigenaHypNH18处理的植物显示,大量的,最高的光合色素和性能,最终导致最高的生物量积累。比较基因组分析和综合基因组挖掘揭示了它们独特的遗传特征,如适应宿主植物的免疫系统和专门的代谢产物。此外,这些菌株含有几个通过营养获取调节直接和间接植物生长促进机制的功能基因,植物激素生产,和缓解压力。实质上,当前的研究认可菌株HypNH10和HypNH18作为微生物辅助的H.perforatum培养的有力候选物,通过突出其独特的基因组特征,这表明他们的一致,兼容性,以及与宿主的多方面有益相互作用,并支持在温室试验中观察到的优异的植物生长促进性能。IMPORTANCE贯叶连翘(圣约翰草)草药制剂是全球治疗抑郁症的最畅销产品之一。整个金丝桃供应的很大一部分来自野生采集,促使他们的自然林分迅速下降。作物种植似乎有利可图,尽管可耕地及其现有的根瘤菌组非常适合传统作物,它的突然引入会造成土壤微生物群失调。此外,传统的植物驯化程序增加了对农用化学品的依赖,可以降低相关根瘤菌的多样性和植物与植物生长促进微生物相互作用的能力,导致不令人满意的作物生产以及有害的环境影响。用与作物相关的有益根际细菌培养穿孔菌可以调和这些担忧。基于体外组合,体内植物促生长试验和植物促生长性状的计算机预测,在这里我们推荐两个与穿孔虫相关的PGPR,KosakoniacowaniiHypNH10和RahnellavariigenaHypNH18,以推断为H.perforatus可持续培养的功能性生物接种剂。
Plants are no longer considered standalone entities; instead, they harbor a diverse community of plant growth-promoting rhizobacteria (PGPR) that aid them in nutrient acquisition and can also deliver resilience. Host plants recognize PGPR in a strain-specific manner; therefore, introducing untargeted PGPR might produce unsatisfactory crop yields. Consequently, to develop a microbe-assisted Hypericum perforatum L. cultivation technique, 31 rhizobacteria were isolated from the plant\'s high-altitude Indian western Himalayan natural habitat and in vitro characterized for multiple plant growth-promoting attributes. Among 31 rhizobacterial isolates, 26 produced 0.59 to 85.29 μg mL-1 indole-3-acetic acid and solubilized 15.77 to 71.43 μg mL-1 inorganic phosphate; 21 produced 63.12 to 99.92% siderophore units, and 15 exhibited 103.60 to 1,296.42 nmol α-ketobutyrate mg-1 protein h-1 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity. Based on superior plant growth-promoting attributes, eight statistically significant multifarious PGPR were further evaluated for an in planta plant growth-promotion assay under poly greenhouse conditions. Plants treated with Kosakonia cowanii HypNH10 and Rahnella variigena HypNH18 showed, by significant amounts, the highest photosynthetic pigments and performance, eventually leading to the highest biomass accumulation. Comparative genome analysis and comprehensive genome mining unraveled their unique genetic features, such as adaptation to the host plant\'s immune system and specialized metabolites. Moreover, the strains harbor several functional genes regulating direct and indirect plant growth-promotion mechanisms through nutrient acquisition, phytohormone production, and stress alleviation. In essence, the current study endorsed strains HypNH10 and HypNH18 as cogent candidates for microbe-assisted H. perforatum cultivation by highlighting their exclusive genomic signatures, which suggest their unison, compatibility, and multifaceted beneficial interactions with their host and support the excellent plant growth-promotion performance observed in the greenhouse trial. IMPORTANCE Hypericum perforatum L. (St. John\'s wort) herbal preparations are among the top-selling products to treat depression worldwide. A significant portion of the overall Hypericum supply is sourced through wild collection, prompting a rapid decline in their natural stands. Crop cultivation seems lucrative, although cultivable land and its existing
rhizomicrobiome are well suited for traditional crops, and its sudden introduction can create soil microbiome dysbiosis. Also, the conventional plant domestication procedures with increased reliance on agrochemicals can reduce the diversity of the associated
rhizomicrobiome and plants\' ability to interact with plant growth-promoting microorganisms, leading to unsatisfactory crop production alongside harmful environmental effects. Cultivating H. perforatum with crop-associated beneficial rhizobacteria can reconcile such concerns. Based on a combinatorial in vitro, in vivo plant growth-promotion assay and in silico prediction of plant growth-promoting traits, here we recommend two H. perforatum-associated PGPR, Kosakonia cowanii HypNH10 and Rahnella variigena HypNH18, to extrapolate as functional bioinoculants for H. perforatum sustainable cultivation.