从患有子宫内膜炎的恒河猴的分泌物中分离并鉴定出细菌。形态学结果表明,该菌株表现为圆形,凸面,在37°C的哥伦比亚血琼脂上培养24小时时,表面光滑且直径为1至2毫米的灰白色菌落;在37°C的沙门氏菌志贺氏菌琼脂(S.S.)上培养24小时,殖民地出现了圆形,扁平,半透明。革兰氏染色显示阴性结果,具有钝端和非孢子形成特征。分子生物学结果表明,该菌株的16SrRNA序列与NCBIGenBank数据库中不同来源的已发表的M.morganii序列的相似性超过96.9%。形态学和分子生物学分析证实,从恒河猴宫颈分泌物中分离的菌株(RM2023)是M.morganii。药敏试验证实分离菌株(RM2023)对头孢曲松敏感,阿米卡星,庆大霉素,头孢唑啉,头孢呋辛,头孢他啶,左氧氟沙星,复方新诺明,诺氟沙星,四环素;对氨苄西林中度敏感;对青霉素耐药,万古霉素,环丙沙星,和克林霉素。研究结果为恒河猴的疾病预防提供了有价值的见解,并有助于分子流行病学研究。
A bacterium was isolated and identified from the secretion of a rhesus monkey with endometritis. The morphological results showed that the strain exhibited round, convex, gray-white colonies with smooth surfaces and diameters ranging from 1 to 2 mm when cultured on Columbia blood agar at 37 °C for 24 h; on salmonella-shigella agar (S.S.) at 37 °C for 24 h, the colonies appeared round, flat, and translucent. Gram staining showed negative results with blunt ends and non-spore-forming characteristics. Molecular biology results showed that the 16S rRNA sequence of the strain revealed over 96.9% similarity with published sequences of M. morganii from different sources in the NCBI GenBank database. Morphological and molecular biology analysis confirmed that the strain (RM2023) isolated from cervical secretions of rhesus monkey was M. morganii. Drug sensitivity testing demonstrated that the isolated strain (RM2023) was sensitive to ceftriaxone, amikacin, gentamicin, cefazolin, cefuroxime, ceftazidime, levofloxacin, cotrimoxazole, norfloxacin, and tetracycline; moderately sensitive to ampicillin; and resistant to penicillin, vancomycin, ciprofloxacin, and clindamycin. The research findings provide valuable insights for disease prevention in rhesus monkeys and contribute to molecular epidemiological studies.