目的:药典规范了用于补充和综合药物不同分支的增强药物制剂的制备。这些制剂的理化性质和生物活性经常在临床前研究中进行调查,然而,目前尚无这方面的实验研究指南。当前的PrePoP指南旨在提供建议,以促进高质量,统计上的声音,和可重复的增强制剂的临床前研究。
方法:使用简化的德尔菲共识方法,从相关科学学会提名的研究人员那里收集了投入,涵盖了该领域基础研究方法的最相关方面,包括适当的控制。样品制备和处理,和统计。经过三轮反馈,最终就对增效制剂进行高质量研究的最重要方面和考虑达成共识。
结果:我们就一系列主题提出了一系列建议,包括实验对照,系统稳定性,盲法和随机化,环境影响,以及制备增强样品和对照的程序,我们解决了这个研究领域的一些具体挑战。
结论:这一专家共识过程产生了一套强大的方法学指南,用于研究增强制剂,并提供了一个有价值的框架,将为这一新兴领域的后续研究提供信息并提高质量。
■TournierAL,BonaminLV,Buchheim-SchmidtS,CartwrightS,DombrowskyC,DoesburgP,HolandinoC,KokornaczykMO,vandeKraatsEB,洛佩斯-卡瓦洛,NandyP,Mazón-SuásteguiJM,MirzajaniF,PoitevinB,ScherrC,小偷K,WürtenbergerS,BaumgartnerS.根据现行药典-PrePoP指南生产的增强制剂的临床前研究的科学指南。JIntegrMed。2024年;Epub提前打印。
OBJECTIVE: Pharmacopoeias regulate the manufacture of potentised pharmaceutical preparations used in different branches of complementary and integrative medicine. The physicochemical properties and biological activity of these preparations are often investigated in preclinical research, yet no guidelines for experimental research currently exist in this area. The present PrePoP guidelines aim to provide recommendations to promote high-quality, statistically sound, and reproducible preclinical research on potentised preparations.
METHODS: Input was gathered from researchers nominated by the relevant scientific societies using a simplified Delphi consensus approach covering the most relevant aspects of basic research methodology in the field including appropriate controls, sample preparation and handling, and statistics. After three rounds of feedback, a consensus was finally reached on the most important aspects and considerations for conducting high-quality research on potentised preparations.
RESULTS: We present a series of recommendations on a range of topics including experimental controls, system stability, blinding and randomisation, environmental influences, and procedures for the preparation of potentised samples and controls, and we address some specific challenges of this research field.
CONCLUSIONS: This expert consensus process resulted in a robust set of methodological guidelines for research on potentised preparations and provides a valuable framework that will inform and improve the quality of subsequent research in this emerging field.
UNASSIGNED: Tournier AL, Bonamin LV, Buchheim-Schmidt S, Cartwright S, Dombrowsky C, Doesburg P, Holandino C, Kokornaczyk MO, van de Kraats EB, López-Carvallo JA, Nandy P, Mazón-Suástegui JM, Mirzajani F, Poitevin B, Scherr C, Thieves K, Würtenberger S, Baumgartner S. Scientific guidelines for preclinical research on potentised preparations manufactured according to current pharmacopoeias-the PrePoP guidelines. J Integr Med. 2024; Epub ahead of print.