OBJECTIVE: Abiotic and biotic components of the environment both limit plant reproduction, but how they interact with one another in combination is less understood. Understanding these interactions is especially relevant because abiotic and biotic environmental components respond differently to various global change drivers. Here we aim to understand whether the effects of pollination (biotic component) on plant reproduction depend on soil moisture (abiotic component), two factors known to affect plant reproduction and that are changing with global change.
METHODS: We conducted pollen supplementation experiments for two plant species, Delphinium nuttallianum and Hydrophyllum fendleri, in subalpine meadows in the Western USA across four years that varied in soil moisture. In a separate one-year field experiment, we factorially crossed water addition with pollen supplementation. We measured proportion fruit set, seeds per fruit, and seeds per plant, in addition to stomatal conductance, to determine whether plant physiology responded to watering.
RESULTS: In the four-year study, only H. fendleri reproduction was pollen limited, and this occurred independently of soil moisture. Experimental water addition significantly increased soil moisture and stomatal conductance for both species. The effect of pollen addition on reproduction depended on the watering treatment only for H. fendleri fruit production. Reproduction in D. nuttallianum was not significantly affected by pollen addition or water addition, but it did respond to interannual variation in soil moisture.
CONCLUSIONS: Although we find some evidence for the effect of a biotic interaction depending on abiotic conditions, it was only for one aspect of reproduction in one species, and it was in an unexpected direction. Our work highlights interactions between the abiotic and biotic components of the environment as an area of further research for improving our understanding of how plant reproduction responds to global change.

Food availability determines the amount of energy animals can acquire and allocate to reproduction and other necessary functions. Female animals that are food limited thus experience reduced energy available for reproduction. When this occurs, females may reduce frequency of reproductive events or the number or size of offspring per reproductive bout. We assessed how maternal diet affects reproductive output in adult female Murray River short-necked turtles, Emydura macquarii, from four wetlands in Victoria. We previously found that turtle diets differ in the composition of plants and animals between our study wetlands. In this study, we tested whether differences in turtle diet composition (i.e. plants and animals) at these wetlands were associated with differences in clutch mass, individual egg mass, bulk egg composition and hatching success. We found total clutch mass increased with maternal body size at each site. At sites where filamentous green algae were scarce and E. macquarii were carnivorous, females produced smaller clutches relative to body size compared to females from sites where algae were abundant, and turtles were more herbivorous. Individual egg mass, bulk egg composition and hatching success did not differ across wetlands. Isotopic analysis revealed significant positive relationships between the carbon and nitrogen isotopes (δ13C, δ15N) of the eggs and those of the mothers, indicating that mothers allocated ratios of carbon and nitrogen isotopes to their eggs similar to those present in their tissues. Our study suggests that at sites where females are more carnivorous due to a relative absence of algae, females produce smaller clutches, but other aspects of their reproduction are not significantly impacted. The reduction in clutch size associated with differences in the availability of dietary plants and animals may have long-term consequences for E. macquarii and other freshwater turtle species that are experiencing population declines.

Predation risk can influence behavior, reproductive investment, and, ultimately, individuals\' fitness. In high-risk environments, females often reduce allocation to reproduction, which can affect offspring phenotype and breeding success. In cooperative breeders, helpers contribute to feed the offspring, and groups often live and forage together. Helpers can, therefore, improve reproductive success, but also influence breeders\' condition, stress levels and predation risk. Yet, whether helper presence can buffer the effects of predation risk on maternal reproductive allocation remains unstudied. Here, we used the cooperatively breeding sociable weaver Philetairus socius to test the interactive effects of predation risk and breeding group size on maternal allocation to clutch size, egg mass, yolk mass, and yolk corticosterone. We increased perceived predation risk before egg laying using playbacks of the adults\' main predator, gabar goshawk (Micronisus gabar). We also tested the interactive effects of group size and prenatal predator playbacks on offspring hatching and fledging probability. Predator-exposed females laid eggs with 4% lighter yolks, but predator-calls\' exposure did not clearly affect clutch size, egg mass, or egg corticosterone levels. Playback-treatment effects on yolk mass were independent of group size, suggesting that helpers\' presence did not mitigate predation risk effects on maternal allocation. Although predator-induced reductions in yolk mass may decrease nutrient availability to offspring, potentially affecting their survival, playback-treatment effects on hatching and fledging success were not evident. The interplay between helper presence and predator effects on maternal reproductive investment is still an overlooked area of life history and physiological evolutionary trade-offs that requires further studies.

A significant advance has been made, especially during the last two decades, in the knowledge of the effects on crustacean species of pollutants proven to be endocrine disruptors in vertebrates. Such effects have been also interpreted in the light of recent studies on crustacean endocrinology. Year after year, the increased number of reports refer to the effects of endocrine disruptors on several processes hormonally controlled. This review is aimed at summarizing and discussing the effects of several kinds of endocrine disruptors on the hormonal control of reproduction (including gonadal growth, sexual differentiation, and offspring development), molting, and intermediate metabolism of crustaceans. A final discussion about the state of the art, as well as the perspective of this toxicological research line is given.

Food is fundamental for the survival of organisms, governing growth, maintenance, and reproduction through the provision of essential macronutrients. However, access to food with optimum macronutrient composition, which will maximize the evolutionary fitness of an organism, is not always guaranteed. This leads to dietary mismatches with potential impacts on organismal performance. To understand the consequences of such dietary mismatches, we examined the effects of isocaloric diets varying in macronutrient composition on eight key organismal traits spanning across the lifespan of a large outbred Drosophila melanogaster population (n ~ 2500). Our findings reveal that carbohydrate-reduced isocaloric diets correlates to accelerated pre-adult development and boosts reproductive output without impacting pre-adult viability and body size. Conversely, an elevated dietary carbohydrate content correlated to reduced lifespan in flies, evidenced by accelerated functional senescence including compromised locomotor activity and deteriorating gut integrity. Furthermore, transcriptomic analysis indicated a substantial difference in gene regulatory landscapes between flies subject to high-carbohydrate versus high-protein diet, with elevated protein levels indicating transcriptomes primed for reduced synthesis of fatty acids. Taken together, our study helps advance our understanding of the effect of macronutrient composition on life history traits and their interrelations, offering critical insights into potential adaptive strategies that organisms might adopt against the continual dietary imbalances prevalent in the rapidly evolving environment.

Reproductive investment, including the number of offspring produced, is one of the fundamental characteristics of a species. It is particularly important for island vertebrates, which face a disproportionate number of threats to their survival, because it predicts, among other things, a species\' resilience to environmental disruption. Taxa producing more offspring recover more quickly from environmental perturbations and survive environmental change better. However, ecologists do not understand which primary drivers shape a species\' reproductive investment well. Here, we compare the reproductive efforts of 14 island populations of the Aegean Wall Lizard (Podarcis erhardii), which lives across widely diverging environmental conditions. We test three hypotheses, namely that reproductive investment (measured as clutch size, clutch volume) is (1) positively associated with predation risk [\'Predation Risk Hypothesis\']; (2) positively associated with the presence of reliable vegetation cover that provides shelter [\'Gravid Female Protection Hypothesis\']; and (3) limited by (and hence positively correlated with) food availability [\'Food Limitation Hypothesis\']. Although field data are somewhat consistent with all three hypotheses, statistical analyses provide strong support for the Predation Risk Hypothesis. The results not only shed light on which fundamental forces shape reproductive investment in island vertebrates, but can also help shape conservation priorities.

Sea turtles are vulnerable to climate change since their reproductive output is influenced by incubating temperatures, with warmer temperatures causing lower hatching success and increased feminization of embryos. Their ability to cope with projected increases in ambient temperatures will depend on their capacity to adapt to shifts in climatic regimes. Here, we assessed the extent to which phenological shifts could mitigate impacts from increases in ambient temperatures (from 1.5 to 3°C in air temperatures and from 1.4 to 2.3°C in sea surface temperatures by 2100 at our sites) on four species of sea turtles, under a \"middle of the road\" scenario (SSP2-4.5). Sand temperatures at sea turtle nesting sites are projected to increase from 0.58 to 4.17°C by 2100 and expected shifts in nesting of 26-43 days earlier will not be sufficient to maintain current incubation temperatures at 7 (29%) of our sites, hatching success rates at 10 (42%) of our sites, with current trends in hatchling sex ratio being able to be maintained at half of the sites. We also calculated the phenological shifts that would be required (both backward for an earlier shift in nesting and forward for a later shift) to keep up with present-day incubation temperatures, hatching success rates, and sex ratios. The required shifts backward in nesting for incubation temperatures ranged from -20 to -191 days, whereas the required shifts forward ranged from +54 to +180 days. However, for half of the sites, no matter the shift the median incubation temperature will always be warmer than the 75th percentile of current ranges. Given that phenological shifts will not be able to ameliorate predicted changes in temperature, hatching success and sex ratio at most sites, turtles may need to use other adaptive responses and/or there is the need to enhance sea turtle resilience to climate warming.

Due to the influence of bio-geographical and environmental factors, as well as anthropogenic landscape features, organisms show different reproductive strategies among different populations. There is a lack of detailed information on the reproductive biology of Kentish plover Charadrius alexandrinus in arid lands in Central Asia. In this study, we summarized the characteristics of the reproductive biology of three geographically distinct plover populations in Aibi Lake in northwestern Xinjiang, Taitema Lake in southern Xinjiang and artificial reservoirs around Urumqi City in northern Xinjiang, based on 440 eggs from 158 nests observed and analyzed from April to July of 2019 and 2020. We found that there was no significant difference in clutch size among the three populations. However, the egg size of the Taitema Lake population was significantly larger than those of the other two populations, whilst the egg volume and clutch volume of the artificial reservoirs\' populations were significantly larger than that of Aibi Lake. With the postponement of laying dates, the northern and northwestern populations showed the characteristics of a small clutch size and a relatively small egg size, respectively, and a decrease in reproductive output. The heavier female plovers in Taitema Lake laid eggs earlier, and there was a significantly positive correlation between female body mass and clutch size and egg size. The tarsometatarsus length of the female plovers was significantly positively correlated with the reproductive output in all three populations. The model selection results show that female body size and ambient temperature restrict the egg size and reproductive output of Kentish plovers, which is consistent with the upper limit hypothesis of the maternal condition and maternal constraint. Our data support the hypothesis that Kentish plovers show distinct flexibility in their breeding strategies to cope with the harsh natural environment in the arid lands of Xinjiang, China. The results of a relatively high average clutch size and average egg size imply that the saline wetlands of Western China are important breeding habitats for Kentish plovers.

The estuarine crab Hemigrapsus crenulatus has a wide distribution range along the coast of the South Pacific Ocean (from 20 °S to 53 S°). This decapod is abundant in these coastal and estuarine habitats and plays a key ecological role as prey of the snook fish (Eleginops maclovinus) and kelp gull (Larus dominicanus). Its diet consists of detritus, dead fish, and crustaceans, and the macroalgae Ulva sp. In response to contrasting environmental conditions and anthropogenic impacts along the Chilean coast, H. crenulatus may present intraspecific variations in its reproductive traits and changes in the elemental composition of its embryos, directly affecting its biological fitness. Along the Chilean coast, female individuals were collected during late spring 2019 and early summer 2020 (from November 2019 to February 2020) in six areas of Chile (north: Tongoy (30°S); south-central: Lenga (36°S), Tubul (37°S); south austral: Calbuco (41°S), Castro (42°S), Quellón (43°S). The environmental conditions (i.e. sea surface temperature, precipitation and chlorophyll α) present during each sampling event were also recorded. We evaluated the reproductive parameters of females (fecundity, reproductive output (RO)) as well as their body size (carapace width and dry weight), volume, water content, dry weight, elemental composition (carbon, hydrogen, nitrogen: CHN), and finally the energy content of their embryos. Our results indicated that the environmental conditions of the sea water temperature, precipitation (proxy of water salinity) and chlorophyll α (proxy of food availability) have direct effects on the reproductive parameters of females and the characteristics of their embryos. We observed a low fecundity and high RO in Calbuco and Quellón, where precipitation was high (i.e. diluted salinity) and temperatures and productivity was low. For embryo traits, the highest values of volume and water content were observed in female crabs from the estuarine areas (i.e. Tongoy, Lenga, Tubul), values much higher than those found in the internal sea of Chile (i.e. Calbuco, Castro, Quellón). For the elemental composition, we observed high nitrogen levels and a low C:N proportion in embryos from female crabs from Lenga (a nitrogen enriched area). Fluctuating environmental variables among localities proved to modulate intraspecific variations in females and embryos of H. crenulatus, revealing different reproductive strategies, particularly in the quality and energy investment per embryo, which subsequently influenced successful embryogenesis and larval survival.

RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously described roles for the highly conserved RBP Caper in neurite morphogenesis in the Drosophila larval peripheral system and in locomotor behavior. However, caper function has not been investigated outside the nervous system, although it is widely expressed in many different tissue types during embryogenesis. Here, we describe novel roles for Caper in fertility and mating behavior. We find that Caper is expressed in ovarian follicles throughout oogenesis but is dispensable for proper patterning of the egg chamber. Additionally, reduced caper function, through either a genetic lesion or RNA interference-mediated knockdown of caper in the female germline, results in females laying significantly fewer eggs than their control counterparts. Moreover, this phenotype is exacerbated with age. caper dysfunction also results in partial embryonic and larval lethality. Given that caper is highly conserved across metazoa, these findings may also be relevant to vertebrates.