背景:环境压力对生殖发育和产量产生负面影响。干旱胁迫,特别是,已在拟南芥生殖发育过程中在形态和转录组水平上进行了检查。然而,生殖发育中不同点的干旱响应转录组变化尚不清楚。此外,在花发育过程中的各个阶段对整个转录组的研究非常感兴趣。
结果:这里,我们处理拟南芥植物水分充足,中度和严重限制的水量时,第一个花达到成熟,并产生RNA-seq数据集的早期,中间,以及在处理后5、6和7天花发育期间的晚期阶段。在不同的干旱条件下,处于不同发育阶段的花朵显示出干旱响应基因(DTG)的差异集,包括那些在不同的GO功能类别中丰富的,如转录调节和对压力的反应(早期),脂质储存(中间阶段),以及花粉和种子的发育和代谢过程(后期)。一些基因家族在不同的花期有不同的成员诱导,这表明相似的生化功能是由不同的成员执行的。在三个花期之间具有差异表达的发育调节基因(DVG)属于在水条件之间相似的GO术语,例如发展和繁殖,新陈代谢和运输,以及信号和应激反应。然而,对于不同的水条件,这种相似的GO术语对应于不同的基因家族或基因家族的不同成员,表明干旱会影响生殖发育过程中不同家庭或家庭成员的表达。在处理后不同天收集的组织的转录组之间的进一步比较鉴定差异基因表达,表明年龄相关基因(ARGs)除了干旱响应和发育外,还可能反映整体植物生理变化。
结论:一起,我们的研究为全球转录组重编程和干旱响应候选基因提供了新的见解,花的发育,这些复杂的生物过程之间的老化和协调。
BACKGROUND: Environmental stresses negatively impact reproductive development and yield. Drought stress, in particular, has been examined during Arabidopsis reproductive development at morphological and transcriptomic levels. However, drought-responsive transcriptomic changes at different points in reproductive development remain unclear. Additionally, an investigation of the entire transcriptome at various stages during flower development is of great interest.
RESULTS: Here, we treat Arabidopsis plants with well-watered and moderately and severely limiting water amounts when the first flowers reach maturity and generate RNA-seq datasets for early, middle, and late phases during flower development at 5, 6, and 7 days following treatment. Under different drought conditions, flowers in different developmental phases display differential sets of drought-responsive genes (DTGs), including those that are enriched in different GO functional categories, such as transcriptional regulation and response to stresses (early phase), lipid storage (middle phase), and pollen and seed development and metabolic processes (late phase). Some gene families have different members induced at different floral phases, suggesting that similar biochemical functions are carried out by distinct members. Developmentally-regulated genes (DVGs) with differential expression among the three floral phases belong to GO terms that are similar between water conditions, such as development and reproduction, metabolism and transport, and signaling and stress response. However, for different water conditions, such similar GO terms correspond to either distinct gene families or different members of a gene family, suggesting that drought affects the expression of distinct families or family members during reproductive development. A further comparison among transcriptomes of tissues collected on different days after treatment identifies differential gene expression, suggesting age-related genes (ARGs) might reflect the changes in the overall plant physiology in addition to drought response and development.
CONCLUSIONS: Together, our study provides new insights into global transcriptome reprogramming and candidate genes for drought response, flower development, aging and coordination among these complex biological processes.