背景:昆虫主要依靠先天免疫抵抗病原体感染。长尾猴(鞘翅目:金雀科),是一种世界性的以水杨酸为食的森林害虫。然而,免疫缺陷途径(IMD)的机制仍然知之甚少。
结果:在这项研究中,我们从转录组分析中获得了一个Relish基因。随后,通过实时定量PCR检测组织和龄表达谱.结果表明,Relish在鸡蛋中具有高表达水平,幼虫和成虫,尤其是在肥胖的身体里。所测试的抗菌肽(AMP)的转录本,dsRelish下调了defensin1,defensin2和attacin2。击倒Relish导致金黄色葡萄球菌感染后幼虫的死亡率更高。此外,我们进行了基于细菌16SrRNA的高通量测序.结果表明,摄入dsRelish后,某些肠道细菌的相对丰度发生了显着变化。
结论:本研究将有助于进一步了解IMD信号通路,促进对P.versicolora的Relish的功能研究。此外,可能会使用Relish作为致死基因来开发遗传害虫管理技术。
BACKGROUND: Insects mainly rely on innate immunity against pathogen infection. Plagiodera versicolora (Coleoptera: Chrysomelidae), is a worldwide leaf-eating forest pest in salicaceous trees. However, the mechanisms behind the immunodeficiency pathway (IMD) remain poorly understood.
RESULTS: In this study, we obtained a
Relish gene from transcriptome analysis. Tissue and instar expression profiles were subsequently obtained using quantitative real-time polymerase chain reaction analysis. The results showed that
Relish has high expression levels in eggs, larvae and adults, and especially in fat bodies. Transcripts of the tested antimicrobial peptides (AMPs), defensin1, defensin2 and attacin2 were downregulated by dsRelish. Knockdown of
Relish led to greater mortality in larvae after Staphylococcus aureus infection. In addition, we performed bacterial 16S ribosomal RNA-based high-throughput sequencing. The results showed that the relative abundance of some gut bacteria was significantly altered after dsRelish ingestion.
CONCLUSIONS: This study provides a greater understanding of the IMD signaling pathway, facilitating functional studies of
Relish in P. versicolora. Moreover, a genetic pest management technique might be developed using
Relish as a lethal gene to control the pest P. versicolora. © 2024 Society of Chemical Industry.